WFRF:(Huang Mingtao 1984)
Sökning: WFRF:(Huang Mingtao 1984) > Balanced traffickin...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04067naa a2200457 4500 | |
| 001 | oai:research.chalmers.se:56e1e011-484f-448a-ac4a-177a94ed8740 | |
| 003 | SwePub | |
| 008 | 180323s2018 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://research.chalmers.se/publication/5015092 URI |
| 024 | 7 | a https://doi.org/10.1186/s13568-018-0571-x2 DOI |
| 040 | a (SwePub)cth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Bao, Jichen,d 1988u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)jichen |
| 245 | 1 0 | a Balanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast |
| 264 | c 2018-03-12 | |
| 264 | 1 | b Springer Science and Business Media LLC,c 2018 |
| 338 | a electronic2 rdacarrier | |
| 520 | a The yeast Saccharomyces cerevisiae is widely used as a cell factory to produce recombinant proteins. However, S. cerevisiae naturally secretes only a few proteins, such as invertase and the mating alpha factor, and its secretory capacity is limited. It has been reported that engineering protein anterograde trafficking from the endoplasmic reticulum to the Golgi apparatus by the moderate overexpression of SEC16 could increase recombinant protein secretion in S. cerevisiae. In this study, the retrograde trafficking in a strain with moderate overexpression of SEC16 was engineered by overexpression of ADP-ribosylation factor GTP activating proteins, Gcs1p and Glo3p, which are involved in the process of COPI-coated vesicle formation. Engineering the retrograde trafficking increased the secretion of α-amylase but did not induce production of reactive oxygen species. An expanded ER membrane was detected in both the GCS1 and GLO3 overexpressio n strains. Physiological characterizations during batch fermentation showed that GLO3 overexpression had better effect on recombinant protein secretion than GCS1 overexpression. Additionally, the GLO3 overexpression strain had higher secretion of two other recombinant proteins, endoglucanase I from Trichoderma reesei and glucan-1,4-α-glucosidase from Rhizopus oryzae, indicating overexpression of GLO3 in a SEC16 moderate overexpression strain might be a general strategy for improving production of secreted proteins by yeast. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Annan industriell bioteknik0 (SwePub)209992 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Other Industrial Biotechnology0 (SwePub)209992 hsv//eng |
| 653 | a Protein secretion | |
| 653 | a Saccharomyces cerevisiae | |
| 653 | a COPI vesicle | |
| 653 | a GLO3 | |
| 653 | a Retrograde trafficking | |
| 700 | 1 | a Huang, Mingtao,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)mingtao |
| 700 | 1 | a Petranovic Nielsen, Dina,d 1975u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)dinap |
| 700 | 1 | a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark4 aut0 (Swepub:cth)nielsenj |
| 710 | 2 | a Chalmers tekniska högskolab Danmarks Tekniske Universitet4 org |
| 773 | 0 | t AMB Expressd : Springer Science and Business Media LLCg 8:1q 8:1x 2191-0855 |
| 856 | 4 | u https://research.chalmers.se/publication/501509/file/501509_Fulltext.pdfx primaryx freey FULLTEXT |
| 856 | 4 | u https://amb-express.springeropen.com/track/pdf/10.1186/s13568-018-0571-x |
| 856 | 4 8 | u https://research.chalmers.se/publication/501509 |
| 856 | 4 8 | u https://doi.org/10.1186/s13568-018-0571-x |
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