WFRF:(Karin Dahlberg)
Sökning: WFRF:(Karin Dahlberg) > (2015-2019) > Microbiota data fro...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04354naa a2200373 4500 | |
| 001 | oai:slubar.slu.se:100914 | |
| 003 | SwePub | |
| 008 | 240910s2019 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://res.slu.se/id/publ/1009142 URI |
| 024 | 7 | a https://doi.org/10.1371/journal.pone.02182572 DOI |
| 040 | a (SwePub)slu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Dahlberg, Josefu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management4 aut0 (Swepub:slu)50106 |
| 245 | 1 0 | a Microbiota data from low biomass milk samples is markedly affected by laboratory and reagent contamination |
| 264 | c 2019-06-13 | |
| 264 | 1 | b Public Library of Science (PLoS),c 2019 |
| 264 | 1 | b Public Library of Science,c 2024 |
| 520 | a Discoveries of bacterial communities in environments that previously have been described as sterile have in recent years radically challenged the view of these environments. In this study we aimed to use 16S rRNA sequencing to describe the composition and temporal stability of the bacterial microbiota in bovine milk from healthy udder quarters, an environment previously believed to be sterile. Sequencing of the 16S rRNA gene is a technique commonly used to describe bacterial composition and diversity in various environments. With the increased use of 16S rRNA gene sequencing, awareness of methodological pitfalls such as biases and contamination has increased although not in equal amount. Evaluation of the composition and temporal stability of the microbiota in 288 milk samples was largely hampered by background contamination, despite careful and aseptic sample processing. Sequencing of no template control samples, positive control samples, with defined levels of bacteria, and 288 milk samples with various levels of bacterial growth, revealed that the data was influenced by contaminating taxa, primarily Methylobacterium. We observed an increasing impact of contamination with decreasing microbial biomass where the contaminating taxa became dominant in samples with less than 10 4 bacterial cells per mL. By applying a contamination filtration on the sequence data, the amount of sequences was substantially reduced but only a minor impact on number of identified taxa and by culture known endogenous taxa was observed. This suggests that data filtration can be useful for identifying biologically relevant associations in milk microbiota data. | |
| 650 | 7 | a LANTBRUKSVETENSKAPERx Veterinärmedicinx Patobiologi0 (SwePub)403022 hsv//swe |
| 650 | 7 | a AGRICULTURAL SCIENCESx Veterinary Sciencex Pathobiology0 (SwePub)403022 hsv//eng |
| 700 | 1 | a Sun, Liu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)51012 |
| 700 | 1 | a Persson Waller, Karinu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,National Veterinary Institute (SVA)4 aut0 (Swepub:slu)48802 |
| 700 | 1 | a Östensson, Karinu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut0 (Swepub:slu)48502 |
| 700 | 1 | a Agenäs, Sigridu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management4 aut0 (Swepub:slu)49929 |
| 700 | 1 | a Dicksved, Johanu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management4 aut0 (Swepub:slu)47518 |
| 710 | 2 | a Sveriges lantbruksuniversitetb Institutionen för husdjurens utfodring och vård (HUV)4 org |
| 710 | 2 | a Sveriges lantbruksuniversitet |
| 773 | 0 | t PLoS ONEd : Public Library of Science (PLoS)g 14q 14x 1932-6203 |
| 856 | 4 | u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0218257&type=printable |
| 856 | 4 8 | u https://res.slu.se/id/publ/100914 |
| 856 | 4 8 | u https://doi.org/10.1371/journal.pone.0218257 |
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