Absolute quantification of human liver metabolite concentrations by localized in vivo 31P NMR spectroscopy in diffuse liver disease

Norén, Bengt, 1955- (author): Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Medicinsk radiologi,Avdelningen för radiologi US

Lundberg, Peter, 1958- (author): Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Medicinsk radiofysik,Radiofysikavdelningen,Centrum för medicinsk bildvetenskap och visualisering, CMIV

Ressner, Marcus, 1967- (author): Linköpings universitet,Tekniska högskolan,Fysiologisk mätteknik,Centrum för medicinsk bildvetenskap och visualisering, CMIV

Wirell, Staffan, 1946- (author): Linköpings universitet,Hälsouniversitetet,Medicinsk radiologi

Almer, Sven, 1953- (author): Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Gastroenterologi och hepatologi,Endokrin- och magtarmmedicinska kliniken US

Smedby, Örjan, 1956- (author): Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Medicinsk radiologi,Avdelningen för radiologi US,Centrum för medicinsk bildvetenskap och visualisering, CMIV

(creator_code:org_t)

2004-09-03
2005
English.
In: European Radiology. - : Springer Science and Business Media LLC. - 0938-7994 .- 1432-1084. ; 15:1, s. 148-157

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Phosphorus-31 NMR spectroscopy using slice selection (DRESS) was used to investigate the absolute concentrations of metabolites in the human liver. Absolute concentrations provide more specific biochemical information compared to spectrum integral ratios. Nine patients with histopathologically proven diffuse liver disease and 12 healthy individuals were examined in a 1.5-T MR scanner (GE Signa LX Echospeed plus). The metabolite concentration quantification procedures included: (1) determination of optimal depth for the in vivo measurements, (2) mapping the detection coil characteristics, (3) calculation of selected slice and liver volume ratios using simple segmentation procedures and (4) spectral analysis in the time domain. The patients had significantly lower concentrations of phosphodiesters (PDE), 6.3±3.9 mM, and ATP-β, 3.6±1.1 mM, (P<0.05) compared with the control group (10.0±4.2 mM and 4.2±0.3 mM, respectively). The concentrations of phosphomonoesters (PME) were higher in the patient group, although this was not significant. Constructing an anabolic charge (AC) based on absolute concentrations, [PME]/([PME] + [PDE]), the patients had a significantly larger AC than the control subjects, 0.29 vs. 0.16 (P<0.005). Absolute concentration measurements of phosphorus metabolites in the liver are feasible using a slice selective sequence, and the technique demonstrates significant differences between patients and healthy subjects.

By the author/editor: Norén, Bengt, 19 ...; Lundberg, Peter, ...; Ressner, Marcus, ...; Wirell, Staffan, ...; Almer, Sven, 195 ...; Smedby, Örjan, 1 ...

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