SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Strand A)
 

Search: WFRF:(Strand A) > (2020-2024) > A European multicen...

  • Hayes, A.University of Oxford (author)

A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • 2020-01-17
  • Wiley,2020
  • 10 s.

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:b7443ef3-5c9b-48a9-9ebb-e36362c994ec
  • https://lup.lub.lu.se/record/b7443ef3-5c9b-48a9-9ebb-e36362c994ecURI
  • https://doi.org/10.1002/jmv.25659DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:142780735URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 µL) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 µL) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 × 10−5). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Nguyen, D.University of Oxford (author)
  • Andersson, M.Oxford University Hospital (author)
  • Antón, A.Vall d'Hebron University Hospital (author)
  • Bailly, J. L.Clermont-Ferrand University Hospital,University of Auvergne (author)
  • Beard, S.Public Health England (author)
  • Benschop, K. S.M.National Institute for Public Health and the Environment (RIVM) (author)
  • Berginc, N.Slovenian National Laboratory of Health, Environment and Food (author)
  • Blomqvist, S.Finnish National Institute for Health and Welfare (author)
  • Cunningham, E.Guy's and St Thomas' NHS Foundation Trust (author)
  • Davis, D.Great Ormond Street Hospital (author)
  • Dembinski, J. L.Norwegian Institute of Public Health (author)
  • Diedrich, S.Robert Koch Institut (author)
  • Dudman, S. G.Norwegian Radium Hospital (author)
  • Dyrdak, R.Karolinska Institutet (author)
  • Eltringham, G. J.A.Freeman Hospital (author)
  • Gonzales-Goggia, S.Public Health England (author)
  • Gunson, R. (author)
  • Howson-Wells, H. C.Queen's Medical Centre, Nottingham (author)
  • Jääskeläinen, A. J.Helsinki University Central Hospital (author)
  • López-Labrador, F. X.Carlos III Health Institute,University of Valencia (author)
  • Maier, M.University Hospital Leipzig (author)
  • Majumdar, M.National Institute for Biological Standards and Control (author)
  • Midgley, S.Danish Serum Institute, Copenhagen (author)
  • Mirand, A.Clermont-Ferrand University Hospital (author)
  • Morley, U.University College Dublin (author)
  • Nordbø, S. A.St. Olav’s University Hospital,Norwegian University of Science and Technology (author)
  • Oikarinen, S.University of Tampere (author)
  • Osman, H.Birmingham Heartlands Hospital (author)
  • Papa, A.Aristotle University of Thessaloniki (author)
  • Pellegrinelli, L.University of Milan (author)
  • Piralla, A.Policlinico San Matteo Pavia Fondazione (author)
  • Rabella, N.Hospital de Sant Pau (author)
  • Richter, J.Cyprus Institute of Neurology and Genetics (author)
  • Smith, M.University of Milan,King's College Hospital (author)
  • Söderlund Strand, A.Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital(Swepub:lu)mikr-aso (author)
  • Templeton, K.Royal Infirmary of Edinburgh (author)
  • Vipond, B.Southmead Hospital (author)
  • Vuorinen, T.Turku University Hospital (author)
  • Williams, C.Royal Oldham Hospital (author)
  • Wollants, E.Catholic University of Leuven (author)
  • Zakikhany, K.Karolinska University Hospital (author)
  • Fischer, T. K.Carlos III Health Institute,Danish Serum Institute, Copenhagen (author)
  • Harvala, H.International Blood Group Reference Laboratory (author)
  • Simmonds, P.University of Oxford (author)
  • University of OxfordOxford University Hospital (creator_code:org_t)

Related titles

  • In:Journal of Medical Virology: Wiley92:8, s. 1065-10740146-66151096-9071

Internet link

Find in a library

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view