Sökning: WFRF:(Wysocki Robert) > The ancillary N-ter...
Fältnamn | Indikatorer | Metadata |
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000 | 03462naa a2200385 4500 | |
001 | oai:gup.ub.gu.se/293969 | |
003 | SwePub | |
008 | 240528s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/2939692 URI |
024 | 7 | a https://doi.org/10.1093/NAR/GKAA3162 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Maciaszczyk-Dziubinska, Ewa4 aut |
245 | 1 0 | a The ancillary N-terminal region of the yeast AP-1 transcription factor Yap8 contributes to its DNA binding specificity. |
264 | 1 | c 2020 |
520 | a Activator protein 1 (AP-1) is one of the largest families of basic leucine zipper (bZIP) transcription factors in eukaryotic cells. How AP-1 proteins achieve target DNA binding specificity remains elusive. In Saccharomyces cerevisiae, the AP-1-like protein (Yap) family comprises eight members (Yap1 to Yap8) that display distinct genomic target sites despite high sequence homology of their DNA binding bZIP domains. In contrast to the other members of the Yap family, which preferentially bind to short (7-8 bp) DNA motifs, Yap8 binds to an unusually long DNA motif (13 bp). It has been unclear what determines this unique specificity of Yap8. In this work, we use molecular and biochemical analyses combined with computer-based structural design and molecular dynamics simulations of Yap8-DNA interactions to better understand the structural basis of DNA binding specificity determinants. We identify specific residues in the N-terminal tail preceding the basic region, which define stable association of Yap8 with its target promoter. We propose that the N-terminal tail directly interacts with DNA and stabilizes Yap8 binding to the 13 bp motif. Thus, beside the core basic region, the adjacent N-terminal region contributes to alternative DNA binding selectivity within the AP-1 family. | |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Fysikalisk kemi0 (SwePub)104022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Physical Chemistry0 (SwePub)104022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
700 | 1 | a Reymer, Anna,d 1983u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology4 aut0 (Swepub:gu)xreyan |
700 | 1 | a Kumar, Nallani Vijayu Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology4 aut |
700 | 1 | a Białek, Wojciech4 aut |
700 | 1 | a Mizio, Katarzyna4 aut |
700 | 1 | a Tamás, Markus J.,d 1970u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology4 aut0 (Swepub:gu)xtamma |
700 | 1 | a Wysocki, Robert4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för kemi och molekylärbiologi4 org |
773 | 0 | t Nucleic acids researchg 48:10, s. 5426-5441q 48:10<5426-5441x 1362-4962 |
856 | 4 8 | u https://gup.ub.gu.se/publication/293969 |
856 | 4 8 | u https://doi.org/10.1093/NAR/GKAA316 |
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