WFRF:(Zhang Wenlong)
Sökning: WFRF:(Zhang Wenlong) > Identification of t...
- Li, QishuangChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences,China Pharmaceutical University (författare)
Identification of the cytochrome P450s responsible for the biosynthesis of two types of aporphine alkaloids and their de novo biosynthesis in yeast
- Artikel/kapitelEngelska2024
Förlag, utgivningsår, omfång ...
- 2024
Nummerbeteckningar
- LIBRIS-ID:oai:research.chalmers.se:7b4241bc-0dce-41df-9e06-9d9bf11bb0e4
- https://research.chalmers.se/publication/541822URI
- https://doi.org/10.1111/jipb.13724DOI
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- Språk:engelska
- Sammanfattning på:engelska
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- Aporphine alkaloids have diverse pharmacological activities; however, our understanding of their biosynthesis is relatively limited. Previous studies have classified aporphine alkaloids into two categories based on the configuration and number of substituents of the D-ring and have proposed preliminary biosynthetic pathways for each category. In this study, we identified two specific cytochrome P450 enzymes (CYP80G6 and CYP80Q5) with distinct activities toward (S)-configured and (R)-configured substrates from the herbaceous perennial vine Stephania tetrandra, shedding light on the biosynthetic mechanisms and stereochemical features of these two aporphine alkaloid categories. Additionally, we characterized two CYP719C enzymes (CYP719C3 and CYP719C4) that catalyzed the formation of the methylenedioxy bridge, an essential pharmacophoric group, on the A- and D-rings, respectively, of aporphine alkaloids. Leveraging the functional characterization of these crucial cytochrome P450 enzymes, we reconstructed the biosynthetic pathways for the two types of aporphine alkaloids in budding yeast (Saccharomyces cerevisiae) for the de novo production of compounds such as (R)-glaziovine, (S)-glaziovine, and magnoflorine. This study provides key insight into the biosynthesis of aporphine alkaloids and lays a foundation for producing these valuable compounds through synthetic biology.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- NATURVETENSKAP Kemi Organisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Organic Chemistry hsv//eng
- engineered yeast
- CYP719C
- aporphine alkaloids
- biosynthesis
- CYP80
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Jiao, Xiang,1990Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)xiangj (författare)
- Li, XinyiChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Shi, WenlongChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Ma, YingChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Tan, XiangmeiChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Gan, JingyiChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Liu, Jimei (författare)
- Yang, JianChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Wang, JianChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Jin, BaolongChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Chen, TongChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Su, P.China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Zhao, YujunChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Zhang, YifengChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Tang, JinfuChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Cui, GuanghongChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Chen, Yun,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)yunc (författare)
- Guo, JuanChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- Huang, LuqiChina Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences (författare)
- China Academy of Chinese Medical Sciences (CACMS)China Pharmaceutical University (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Journal of Integrative Plant BiologyIn Press1672-90721744-7909
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