Oxygen Uptake Rate (OUR) tests for assessment of nitrifying activities in the deammonification system

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QC 20101108
The two-step deammonification system for nitrogen removal was established in a technical-scale pilot plant with moving-bed reactors with Kaldnes rings as biofilm carriers. The study consisted in observing the presence of nitrifiers in both activated sludge and biofilm in the partial nitritation reactor and in the Anammox reactor. Oxygen Uptake Rate (OUR) tests were used as a tool to assess qualitatively and quantitatively ammonia- and nitrite-oxidizing bacteria as well as heterotrophic activity. Thirteen series of OUR tests focussed on monitoring the activity of nitrifying microorganisms in the deammonification system. It was demonstrated that the nitrifying culture mostly forms biofilm in the partial nitritation reactor. The results confirmed that there is only insignificant nitrifying activity present in the Anammox reactor and it is mainly concentrated in the activated sludge. It is most likely that nitrifiers sustain oxygen-limited conditions for the Anammox bacteria. The moving-bed system is therefore appropriate to obtain efficient removal of nitrogen with taking advantage of the cooperation of many bacterial cultures.

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