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Validation of a scr...
Validation of a screening method for the detection of colistin-resistant E. coli containing mcr-1 in feral swine feces
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- Chandler, Jeffrey C. (författare)
- USDA, CO 80526 USA
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- Franklin, Alan B. (författare)
- USDA, CO 80526 USA
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- Bevins, Sarah N. (författare)
- USDA, CO 80526 USA
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- Bentler, Kevin T. (författare)
- USDA, CO 80526 USA
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- Bonnedahl, Jonas (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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- Ahlstrom, Christina A. (författare)
- US Geol Survey, AK USA
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- Bisha, Bledar (författare)
- Univ Wyoming, WY 82071 USA
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- Shriner, Susan A. (författare)
- USDA, CO 80526 USA
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(creator_code:org_t)
- ELSEVIER, 2020
- 2020
- Engelska.
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Ingår i: Journal of Microbiological Methods. - : ELSEVIER. - 0167-7012 .- 1872-8359. ; 172
- Relaterad länk:
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https://digitalcommo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A method was developed and validated for the detection of colistin-resistant Escherichia coli containing mcr-1 in the feces of feral swine. Following optimization of an enrichment method using EC broth supplemented with colistin (1 mu g/mL) and vancomycin (8 mu g/mL), aliquots derived from 100 feral swine fecal samples were spiked with of one of five different mcr-1 positive E. coli strains (between 10(0) and 10(4) CFU/g), for a total of 1110 samples tested. Enrichments were then screened using a simple boil-prep and a previously developed real-time PCR assay for mcr-1 detection. The sensitivity of the method was determined in swine feces, with mcr-1 E. coli inocula of 0.1-9.99 CFU/g (n = 340), 10-49.99 CFU/g (n = 170), 50-99 CFU/g (n = 255), 100-149 CFU/g (n = 60), and 200-2200 CFU/g (n = 175), which were detected with 32%, 72%, 88%, 95%, and 98% accuracy, respectively. Uninoculated controls (n = 100) were negative for mcr-1 following enrichment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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- ref (ämneskategori)
- art (ämneskategori)
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