Programmed Cell Death Ligand 1 Immunohistochemistry: A Concordance Study Between Surgical Specimen, Biopsy, and Tissue Microarray

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Elfving, HedvigUppsala universitet,Klinisk och experimentell patologi,Patrick Micke (författare)

Programmed Cell Death Ligand 1 Immunohistochemistry : A Concordance Study Between Surgical Specimen, Biopsy, and Tissue Microarray

Artikel/kapitelEngelska2019

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Elsevier BV,2019
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LIBRIS-ID:oai:DiVA.org:uu-390976
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-390976URI
https://doi.org/10.1016/j.cllc.2019.02.012DOI

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Språk:engelska
Sammanfattning på:engelska

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Programmed cell death ligand 1 (PD-L1) expression within the same lung cancer tissue is variable. In this study we evaluated if the PD-L1 expression on small biopsy specimens represent the PD-L1 status of the corresponding resection specimen. Our results indicate a relative good agreement between biopsy and surgical specimens, with a discordance in approximately 10% of the cases. Background: The immunohistochemical analysis of programmed cell death ligand 1 (PD-L1) expression in tumor tissue of non-small-cell lung cancer patients has now been integrated in the diagnostic workup. Analysis is commonly done on small tissue biopsy samples representing a minimal fraction of the whole tumor. The aim of the study was to evaluate the correlation of PD-L1 expression on biopsy specimens with corresponding resection specimens. Materials and Methods: In total, 58 consecutive cases with preoperative biopsy and resected tumor specimens were selected. From each resection specimen 2 tumor cores were compiled into a tissue microarray (TMA). Immunohistochemical staining with the antibody SP263 was performed on biopsy specimens, resection specimens (whole sections), as well as on the TMA. Results: The proportion of PD-L1-positive stainings were comparable between the resection specimens (48% and 19%), the biopsies (43% and 17%), and the TMAs (47% and 14%), using cutoffs of 1% and 50%, respectively (P > .39 all comparisons). When the resection specimens were considered as reference, PD-L1 status differed in 16%/5% for biopsies and in 9%/9% for TMAs (1%/50% cutoff). The sensitivity of the biopsy analysis was 79%/82% and the specificity was 90%/98% at the 1%/50% cutoff. The Cohens kappa value for the agreement between biopsy and tumor. was 0.70 at the 1% cutoff and 0.83 at the 50% cutoff. Conclusion: The results indicate a moderate concordance between the analysis of biopsy and whole tumor tissue, resulting in misclassification of samples in particular when the lower 1% cutoff was used. Clinicians should be aware of this uncertainty when interpreting PD-L1 reports for treatment decisions.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Klinisk laboratoriemedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Clinical Laboratory Medicine hsv//eng
Checkpoint inhibitors
Nivolumab
PD-1
PD-L1
Pembrolizumab
Patologi
Pathology

Biuppslag (personer, institutioner, konferenser, titlar ...)

Mattsson, Johanna Sofia Margareta,1985-Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke(Swepub:uu)johma961 (författare)
Lindskog, CeciliaUppsala universitet,Klinisk och experimentell patologi(Swepub:uu)cecli129 (författare)
Backman, MaxUppsala universitet,Klinisk och experimentell patologi,Patrick Micke(Swepub:uu)maxba655 (författare)
Menzel, UweUppsala universitet,Klinisk och experimentell patologi(Swepub:uu)uwemenze (författare)
Micke, PatrickUppsala universitet,Klinisk och experimentell patologi,Patrick Micke(Swepub:uu)patmi676 (författare)
Uppsala universitetKlinisk och experimentell patologi (creator_code:org_t)

Sammanhörande titlar

Ingår i:Clinical Lung Cancer: Elsevier BV20:4, s. 258-262.e11525-73041938-0690

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Av författaren/redakt...: Elfving, Hedvig; Mattsson, Johann ...; Lindskog, Cecili ...; Backman, Max; Menzel, Uwe; Micke, Patrick

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Klinisk laborato ...

Artiklar i publikationen: Clinical Lung Ca ...

Av lärosätet: Uppsala universitet

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