Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.

Schaller, Hubert (author)
Laux, Valerie (author)
Haertlein, Michael (author)
Sferrazza, Michele (author)
Marechal, Eric (author)
Wacklin, HannaLund University,Lunds universitet,European Spallation Source ESS AB,Stiftelser och övriga anknutna verksamheter,Other Institutions and Utilities(Swepub:lu)esss-haw (author)
Jouhet, Juliette (author)
Fragneto, Giovanna (author)
European Spallation Source ESS ABStiftelser och övriga anknutna verksamheter (creator_code:org_t)

By the author/editor: de Ghellinck, Al ...; Schaller, Hubert; Laux, Valerie; Haertlein, Micha ...; Sferrazza, Miche ...; Marechal, Eric; show more...; Wacklin, Hanna; Jouhet, Juliette; Fragneto, Giovan ...; show less...

About the subject

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.