WFRF:(Fragneto Giovanna)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02707naa a2200397 4500 | |
| 001 | oai:lup.lub.lu.se:63f8b527-fbca-4bd9-aa79-21ba97030dbf | |
| 003 | SwePub | |
| 008 | 160401s2014 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/44871752 URI |
| 024 | 7 | a https://doi.org/10.1371/journal.pone.00929992 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a de Ghellinck, Alexis4 aut |
| 245 | 1 0 | a Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
| 264 | c 2014-04-18 | |
| 264 | 1 | b Public Library of Science (PLoS),c 2014 |
| 520 | a Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. | |
| 650 | 7 | a NATURVETENSKAPx Fysik0 (SwePub)1032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Physical Sciences0 (SwePub)1032 hsv//eng |
| 700 | 1 | a Schaller, Hubert4 aut |
| 700 | 1 | a Laux, Valerie4 aut |
| 700 | 1 | a Haertlein, Michael4 aut |
| 700 | 1 | a Sferrazza, Michele4 aut |
| 700 | 1 | a Marechal, Eric4 aut |
| 700 | 1 | a Wacklin, Hannau Lund University,Lunds universitet,European Spallation Source ESS AB,Stiftelser och övriga anknutna verksamheter,Other Institutions and Utilities4 aut0 (Swepub:lu)esss-haw |
| 700 | 1 | a Jouhet, Juliette4 aut |
| 700 | 1 | a Fragneto, Giovanna4 aut |
| 710 | 2 | a European Spallation Source ESS ABb Stiftelser och övriga anknutna verksamheter4 org |
| 773 | 0 | t PLoS ONEd : Public Library of Science (PLoS)g 9:4q 9:4x 1932-6203 |
| 856 | 4 | u http://dx.doi.org/10.1371/journal.pone.0092999x freey FULLTEXT |
| 856 | 4 | u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092999&type=printable |
| 856 | 4 8 | u https://lup.lub.lu.se/record/4487175 |
| 856 | 4 8 | u https://doi.org/10.1371/journal.pone.0092999 |
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