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Sökning: WFRF:(Haavardsholm EA) > MicroRNA Expression...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003551naa a2200421 4500
001oai:prod.swepub.kib.ki.se:146463605
003SwePub
008240811s2021 | |||||||||||000 ||eng|
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1464636052 URI
0247 a https://doi.org/10.3389/fimmu.2021.6637362 DOI
040 a (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Heinicke, F4 aut
2451 0a MicroRNA Expression Differences in Blood-Derived CD19+ B Cells of Methotrexate Treated Rheumatoid Arthritis Patients
264 c 2021-04-09
264 1b Frontiers Media SA,c 2021
520 a Rheumatoid arthritis (RA) is a complex disease with a wide range of underlying susceptibility factors. Recently, dysregulation of microRNAs (miRNAs) in RA have been reported in several immune cell types from blood. However, B cells have not been studied in detail yet. Given the autoimmune nature of RA with the presence of autoantibodies, CD19+ B cells are a key cell type in RA pathogenesis and alterations in CD19+ B cell subpopulations have been observed in patient blood. Therefore, we aimed to reveal the global miRNA repertoire and to analyze miRNA expression profile differences in homogenous RA patient phenotypes in blood-derived CD19+ B cells. Small RNA sequencing was performed on CD19+ B cells of newly diagnosed untreated RA patients (n=10), successfully methotrexate (MTX) treated RA patients in remission (MTX treated RA patients, n=18) and healthy controls (n=9). The majority of miRNAs was detected across all phenotypes. However, significant expression differences between MTX treated RA patients and controls were observed for 27 miRNAs, while no significant differences were seen between the newly diagnosed patients and controls. Several of the differentially expressed miRNAs were previously found to be dysregulated in RA including miR-223-3p, miR-486-3p and miR-23a-3p. MiRNA target enrichment analysis, using the differentially expressed miRNAs and miRNA-target interactions from miRTarBase as input, revealed enriched target genes known to play important roles in B cell activation, differentiation and B cell receptor signaling, such as STAT3, PRDM1 and PTEN. Interestingly, many of those genes showed a high degree of correlated expression in CD19+ B cells in contrast to other immune cell types. Our results suggest important regulatory functions of miRNAs in blood-derived CD19+ B cells of MTX treated RA patients and motivate for future studies investigating the interactive mechanisms between miRNA and gene targets, as well as the possible predictive power of miRNAs for RA treatment response.
7001 a Zhong, XFu Karolinska Institutet4 aut
7001 a Flam, ST4 aut
7001 a Breidenbach, J4 aut
7001 a Leithaug, M4 aut
7001 a Maehlen, MT4 aut
7001 a Lillegraven, S4 aut
7001 a Aga, AB4 aut
7001 a Norli, ES4 aut
7001 a Mjaavatten, MD4 aut
7001 a Haavardsholm, EA4 aut
7001 a Zucknick, M4 aut
7001 a Rayner, S4 aut
7001 a Lie, BA4 aut
7102 a Karolinska Institutet4 org
7730 t Frontiers in immunologyd : Frontiers Media SAg 12, s. 663736-q 12<663736-x 1664-3224
8564 u https://www.frontiersin.org/articles/10.3389/fimmu.2021.663736/pdf
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:146463605
8564 8u https://doi.org/10.3389/fimmu.2021.663736

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