WFRF:(Huang Mingtao 1984)
Sökning: WFRF:(Huang Mingtao 1984) > Moderate Expression...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03530naa a2200385 4500 | |
| 001 | oai:research.chalmers.se:70d5f7a6-c0d9-4d30-8935-3527f05aab25 | |
| 003 | SwePub | |
| 008 | 171007s2017 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://doi.org/10.1128/AEM.03400-162 DOI |
| 024 | 7 | a https://research.chalmers.se/publication/2511142 URI |
| 040 | a (SwePub)cth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Bao, Jichen,d 1988u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)jichen |
| 245 | 1 0 | a Moderate Expression of SEC16 Increases Protein Secretion by Saccharomyces cerevisiae |
| 264 | 1 | c 2017 |
| 338 | a electronic2 rdacarrier | |
| 520 | a The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of the secretory pathway may reduce its productivity. Here, we increased the secretion of a heterologous beta-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing SEC16, which is involved in protein translocation from the endoplasmic reticulum to the Golgi apparatus. The moderate overexpression of SEC16 increased beta-amylase secretion by generating more endoplasmic reticulum exit sites. The production of reactive oxygen species resulting from the heterologous beta-amylase production was reduced. A genome-wide expression analysis indicated decreased endoplasmic reticulum stress in the strain that moderately overexpressed SEC16, which was consistent with a decreased volume of the endoplasmic reticulum. Additionally, fewer mitochondria were observed. Finally, the moderate overexpression of SEC16 was shown to improve the secretion of two other recombinant proteins, Trichoderma reesei endoglucanase I and Rhizopus oryzae glucan-1,4-beta-glucosidase, indicating that this mechanism is of general relevance. IMPORTANCE There is an increasing demand for recombinant proteins to be used as enzymes and pharmaceuticals. The yeast Saccharomyces cerevisiae is a cell factory that is widely used to produce recombinant proteins. Our study revealed that moderate overexpression of SEC16 increased recombinant protein secretion in S. cerevisiae. This new strategy can be combined with other targets to engineer cell factories to efficiently produce protein in the future. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
| 653 | a SEC16 | |
| 653 | a protein secretion | |
| 653 | a ERES | |
| 653 | a mitochondria | |
| 653 | a ROS | |
| 700 | 1 | a Huang, Mingtao,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)mingtao |
| 700 | 1 | a Petranovic Nielsen, Dina,d 1975u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)dinap |
| 700 | 1 | a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj |
| 710 | 2 | a Chalmers tekniska högskola4 org |
| 773 | 0 | t Applied and Environmental Microbiologyg 83:14, s. Article no. UNSP e03400-16q 83:14<Article no. UNSP e03400-16x 1098-5336x 0099-2240 |
| 856 | 4 | u http://publications.lib.chalmers.se/records/fulltext/251114/local_251114.pdfx primaryx freey FULLTEXT |
| 856 | 4 8 | u https://doi.org/10.1128/AEM.03400-16 |
| 856 | 4 8 | u https://research.chalmers.se/publication/251114 |
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