KODFISH, a direct RNA targeted, single nucleotide sensitive in situ transcriptomics.

• In-situ detection of expressed point mutations within fixed cells or tissues is challenging due to the low sensitivity or poor specificity of available molecular tools. Here, we present KODFISH, an efficient and specific approach based on single nucleotide sensitive RNA-based in-situ sequencing (RNA-ISS) using padlock probes and rolling circle amplification. KODFISH enables the accurate in-situ discrimination of single nucleotide changes, allowing the spatial detection of expressed mutations with higher sensitivity and specificity than currently available methods.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

in situ hybridization

in situ sequencing

spatial transcriptomics

biokemi

Biochemistry

Publikations- och innehållstyp

vet (ämneskategori)

ovr (ämneskategori)