L773:1368 8375 OR L773:1879 0593
Sökning: L773:1368 8375 OR L773:1879 0593 > Matrix metalloprote...
Matrix metalloproteinase-7 and -13 expression associate to cisplatin resistance in head and neck cancer cell lines.
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- Ansell, Anna (författare)
- Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet
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- Jerhammar, Fredrik (författare)
- Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet
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- Ceder, Rebecca (författare)
- Karolinska Institutet
- visa fler...
- visa färre...
- (creator_code:org_t)
- Elsevier, 2009
- 2009
- Engelska.
- Ingår i: Oral Oncology. - : Elsevier. - 1368-8375 .- 1879-0593. ; 45:10, s. 866-871
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Concomitant chemoradiotherapy is a common treatment for advanced head and neck squamous cell carcinomas (HNSCC). Cisplatin is the backbone of chemotherapy regimens used to treat HNSCC. Therefore, the aim of this study was to identify predictive markers for cisplatin treatment outcome in HNSCC. The intrinsic cisplatin sensitivity (ICS) was determined in a panel of tumour cell lines. From this panel, one sensitive and two resistant cell lines were selected for comparative transcript profiling using microarray analysis. The enrichment of Gene Ontology (GO) categories in sensitive versus resistant cell lines were assessed using the Gene Ontology Tree Machine bioinformatics tool. In total, 781 transcripts were found to be differentially expressed and 11 GO categories were enriched. Transcripts contributing to this enrichment were further analyzed using Ingenuity Pathway Analysis (IPA) for identification of key regulator genes. IPA recognized 20 key regulator genes of which five were differentially expressed in sensitive versus resistant cell lines. The mRNA level of these five genes was further assessed in a panel of 25 HNSCC cell lines using quantitative real-time PCR. Among these key regulators, MMP-7 and MMP-13 are implicated as potential biomarkers of ICS. Taken together, genome-wide transcriptional analysis identified single genes, GO categories as well as molecular networks that are differentially expressed in HNSCC cell lines with different ICS. Furthermore, two novel predictive biomarkers for cisplatin resistance, MMP-7 and MMP-13, were identified.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Predictive markers; Gene Ontology; Head and neck cancer; Cisplatin; Microarray; MMPs
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
- Ansell, Anna
- Jerhammar, Fredr ...
- Ceder, Rebecca
- Grafström, Rolan ...
- Grénman, Reidar
- Roberg, Karin
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Cancer och onkol ...
- Artiklar i publikationen
- Oral Oncology
- Av lärosätet
- Linköpings universitet
- Karolinska Institutet
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