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Plasma ACE2 species are differentially altered in COVID-19 patients
- Garcia-Ayllon, M. S. (författare)
- Moreno-Perez, O. (författare)
- Garcia-Arriaza, J. (författare)
- visa fler...
- Ramos-Rincon, J. M. (författare)
- Cortes-Gomez, M. A. (författare)
- Andres, M. (författare)
- Leon-Ramirez, J. M. (författare)
- Boix, V. (författare)
- Gil, J. (författare)
- Esteban, M. (författare)
- Merino, E. (författare)
- Saez-Valero, J. (författare)
- visa färre...
- (creator_code:org_t)
- 2021
- 2021
- Engelska.
- Ingår i: FASEB Journal. - 0892-6638. ; 35:8
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Studies are needed to identify useful biomarkers to assess the severity and prognosis of COVID-19 disease, caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) virus. Here, we examine the levels of various plasma species of the SARS-CoV-2 host receptor, the angiotensin-converting enzyme 2 (ACE2), in patients at different phases of the infection. Human plasma ACE2 species were characterized by immunoprecipitation and western blotting employing antibodies against the ectodomain and the C-terminal domain, using a recombinant human ACE2 protein as control. In addition, changes in the cleaved and full-length ACE2 species were also examined in serum samples derived from humanized K18-hACE2 mice challenged with a lethal dose of SARS-CoV-2. ACE2 immunoreactivity was present in human plasma as several molecular mass species that probably comprise truncated (70 and 75 kDa) and full-length forms (95, 100, 130, and 170 kDa). COVID-19 patients in the acute phase of infection (n = 46) had significantly decreased levels of ACE2 full-length species, while a truncated 70-kDa form was marginally higher compared with non-disease controls (n = 26). Levels of ACE2 full-length species were in the normal range in patients after a recovery period with an interval of 58-70 days (n = 29), while the 70-kDa species decreased. Levels of the truncated ACE2 species served to discriminate between individuals infected by SARS-CoV-2 and those infected with influenza A virus (n = 17). In conclusion, specific plasma ACE2 species are altered in patients with COVID-19 and these changes normalize during the recovery phase. Alterations in ACE2 species following SARS-CoV-2 infection warrant further investigation regarding their potential usefulness as biomarkers for the disease process and to asses efficacy during vaccination.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- ACE2
- biomarker
- COVID-19
- plasma
- SARS-CoV-2
- angiotensin-converting enzyme
- sars-coronavirus
- functional receptor
- messenger-rna
- spike protein
- expression
- cov
- sars-cov-2
- tmprss2
- cloning
- Biochemistry & Molecular Biology
- Life Sciences & Biomedicine - Other
- Topics
- Cell Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Garcia-Ayllon, M ...
- Moreno-Perez, O.
- Garcia-Arriaza, ...
- Ramos-Rincon, J. ...
- Cortes-Gomez, M. ...
- Brinkmalm, Gunna ...
- visa fler...
- Andres, M.
- Leon-Ramirez, J. ...
- Boix, V.
- Gil, J.
- Zetterberg, Henr ...
- Esteban, M.
- Merino, E.
- Saez-Valero, J.
- visa färre...
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