WFRF:(Wakamatsu Kazumasa)
Sökning: WFRF:(Wakamatsu Kazumasa) > HPLC analysis of ph...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03717naa a2200361 4500 | |
| 001 | oai:DiVA.org:liu-21494 | |
| 003 | SwePub | |
| 008 | 091002s2003 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-214942 URI |
| 024 | 7 | a https://doi.org/10.1034/j.1600-0749.2003.00086.x2 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Takasaki, Akihikou Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan4 aut |
| 245 | 1 0 | a HPLC analysis of pheomelanin degradation products in human urine |
| 264 | c 2003-11-17 | |
| 264 | 1 | b Wiley,c 2003 |
| 338 | a print2 rdacarrier | |
| 520 | a A sensitive and specific high performance liquid chromatography (HPLC) method was developed to quantify 4-amino-3-hydroxyphenylalanine (4-AHP) and 3-amino-4-hydroxyphenylalanine (3-AHP) in urine. In degradation studies of melanin pigment, 4-AHP and 3-AHP are derived from benzothiazine units of pheomelanin and pheomelanin-related metabolites such as trichochromes. 5-S-Cysteinyldopa-derived benzothiazine products give 4-AHP while 2-S-cysteinyldopa-derived benzothiazine products give 3-AHP. 3-AHP is also derived from nitrotyrosine formed by nitration of tyrosine with reactive nitrogen species. For this reason, the influence of this biological process on the amount of 3-AHP found in biological material have been investigated. The method is based on hydriodic acid hydrolysis of the melanin polymer and reversed-phase HPLC with electrochemical detection of the degradation products 4-AHP and 3-AHP. The mobile phase consists of 25 mM ammonium acetate and sodium octanesulfonate as an ion-pairing reagent. The 4-AHP and 3-AHP peaks were well separated and the detector response was linear within the range 0-2 ng injected for both compounds. With the developed chromatographic system, 4-AHP and 3-AHP showed good separation in the biological samples. There was a strong correlation between 4-AHP and 3-AHP in the urine of 50 malignant melanoma patients and two healthy subjects (R0.977). The two compounds were also strongly correlated with 5-S-cysteinyldopa in urine, the correlation coefficients being 0.862 and 0.907, respectively. The method described is sensitive enough for analysis of pheomelanin in urine and in several other biological samples. The results indicate that 3-AHP in urine is not influenced by excreted 3-nitrotyrosine and the data indicate that pheomelanins are excreted in the urine of melanoma patients. | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Nezirevic Dernroth, Dzenetau Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)dzene44 |
| 700 | 1 | a Årstrand, Kerstinu Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)kerar67 |
| 700 | 1 | a Wakamatsu, Kazumasau Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan4 aut |
| 700 | 1 | a Ito, Shosukeu Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan4 aut |
| 700 | 1 | a Kågedal, Bertilu Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)berka70 |
| 710 | 2 | a Fujita Health University School of Health Sciences, Toyoake, Aichi, Japanb Klinisk kemi4 org |
| 773 | 0 | t Pigment Cell Researchd : Wileyg 16:5, s. 480-486q 16:5<480-486x 0893-5785x 1600-0749 |
| 856 | 4 | u http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-21486y Link to Ph.D. Thesis |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-21494 |
| 856 | 4 8 | u https://doi.org/10.1034/j.1600-0749.2003.00086.x |
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