WFRF:(Aguirre Javier)
Sökning: WFRF:(Aguirre Javier) > Real-time measureme...
LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03715naa a2200457 4500 | |
| 001 | oai:DiVA.org:uu-440887 | |
| 003 | SwePub | |
| 008 | 210428s2021 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4408872 URI |
| 024 | 7 | a https://doi.org/10.1073/pnas.20133151182 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Aguirre Rivera, Javier,d 1989-u Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)javag704 |
| 245 | 1 0 | a Real-time measurements of aminoglycoside effects on protein synthesis in live cells |
| 264 | c 2021-02-22 | |
| 264 | 1 | b Proceedings of the National Academy of Sciences (PNAS),c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a The spread of antibiotic resistance is turning many of the currently used antibiotics less effective against common infections. To address this public health challenge, it is critical to enhance our understanding of the mechanisms of action of these compounds. Aminoglycoside drugs bind the bacterial ribosome, and decades of results from in vitro biochemical and structural approaches suggest that these drugs disrupt protein synthesis by inhibiting the ribosome's translocation on the messenger RNA, as well as by inducing miscoding errors. So far, however, we have sparse information about the dynamic effects of these compounds on protein synthesis inside the cell. In the present study, we measured the effect of the aminoglycosides apramycin, gentamicin, and paromomycin on ongoing protein synthesis directly in live Escherichia coli cells by tracking the binding of dye-labeled transfer RNAs to ribosomes. Our results suggest that the drugs slow down translation elongation two- to fourfold in general, and the number of elongation cycles per initiation event seems to decrease to the same extent. Hence, our results imply that none of the drugs used in this study cause severe inhibition of translocation. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a translation | |
| 653 | a antibiotics | |
| 653 | a single-molecule tracking | |
| 653 | a superresolution | |
| 653 | a microscopy | |
| 653 | a tRNA | |
| 700 | 1 | a Larsson, Jimmy,d 1977-u Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)jilar813 |
| 700 | 1 | a Volkov, Ivanu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)ivavo256 |
| 700 | 1 | a Seefeldt, A. Carolinu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)carse696 |
| 700 | 1 | a Sanyal, Suparnau Uppsala universitet,Teknisk-naturvetenskapliga fakulteten,Molekylärbiologi4 aut0 (Swepub:uu)sucsa102 |
| 700 | 1 | a Johansson, Magnusu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)majoh087 |
| 710 | 2 | a Uppsala universitetb Molekylär systembiologi4 org |
| 773 | 0 | t Proceedings of the National Academy of Sciences of the United States of Americad : Proceedings of the National Academy of Sciences (PNAS)g 118:9q 118:9x 0027-8424x 1091-6490 |
| 856 | 4 | u https://doi.org/10.1073/pnas.2013315118y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1547978/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://www.pnas.org/content/pnas/118/9/e2013315118.full.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-440887 |
| 856 | 4 8 | u https://doi.org/10.1073/pnas.2013315118 |
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