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Lack of the light-harvesting complex CP24 affects the structure and function of the grana membranes of higher plant chloroplasts.
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- Kovács, Laszlo (author)
- Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
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- Damkjaer, Jakob, 1976- (author)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Kereïche, Sami (author)
- Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
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- (creator_code:org_t)
- 2006-11-17
- 2006
- English.
- In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 18:11, s. 3106-20
- Journal article (peer-reviewed)
Abstract
Subject headings
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- The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana plants depleted of one of these, the CP24 light-harvesting complex, have been analyzed. CP24-deficient plants showed a decrease in light-limited photosynthetic rate and growth, but the pigment and protein content of the thylakoid membranes were otherwise almost unchanged. However, there was a major change in the macroorganization of PSII within these membranes; electron microscopy and image analysis revealed the complete absence of the C2S2M2 light-harvesting complex II (LHCII)/PSII supercomplex predominant in wild-type plants. Instead, only C2S2 supercomplexes, which are deficient in the LHCIIb M-trimers, were found. Spectroscopic analysis confirmed the disruption of the wild-type macroorganization of PSII. It was found that the functions of the PSII antenna were disturbed: connectivity between PSII centers was reduced, and maximum photochemical yield was lowered; rapidly reversible nonphotochemical quenching was inhibited; and the state transitions were altered kinetically. CP24 is therefore an important factor in determining the structure and function of the PSII light-harvesting antenna, providing the linker for association of the M-trimer into the PSII complex, allowing a specific macroorganization that is necessary both for maximum quantum efficiency and for photoprotective dissipation of excess excitation energy.
Keyword
- Arabidopsis/*metabolism/radiation effects/ultrastructure
- Arabidopsis Proteins/analysis/isolation & purification/*metabolism
- Chloroplasts/*ultrastructure
- Chromatography; Gel
- Circular Dichroism
- DNA; Bacterial/metabolism
- Fluorescence
- Light
- Light-Harvesting Protein Complexes/analysis/*deficiency/isolation & purification
- Models; Biological
- Mutagenesis; Insertional
- Photosynthesis/radiation effects
- Photosynthetic Reaction Center Complex Proteins/metabolism
- Photosystem II Protein Complex/metabolism
- Pigments; Biological/metabolism
- Plant Leaves/radiation effects
- RNA; Antisense/metabolism
- Structure-Activity Relationship
- Temperature
- Thylakoids/*ultrastructure
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- ref (subject category)
- art (subject category)
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- Horton, Peter
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