Sökning: WFRF:(Li Xiaoli) >
Maternal high-prote...
Maternal high-protein diet modulates hepatic growth axis in weaning piglets by reprogramming the IGFBP-3 gene
-
- Cong, Rihua (författare)
- Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
-
- Qu, Xiaoli (författare)
- Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China
-
- Zhang, Hui (författare)
- Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
-
visa fler...
-
- Hu, Yongling (författare)
- Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
-
- Ye, Silin (författare)
- Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
-
- Cai, Demin (författare)
- Univ Calif Davis, Dept Biochem & Mol Med, Sacramento, CA 95817 USA.;Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
-
- Li, Xian (författare)
- Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
-
- Liu, Haoyu (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
visa färre...
-
(creator_code:org_t)
- 2019-09-30
- 2020
- Engelska.
-
Ingår i: European Journal of Nutrition. - : Springer. - 1436-6207 .- 1436-6215. ; 59:6, s. 2497-2506
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://link.springe...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- PurposeThe aim of this study was to investigate the effects of maternal high dietary protein intake on the hepatic growth axis in offspring.MethodsFourteen primiparous purebred Meishan sows were fed either a standard-protein (SP, n = 7) diet or a high-protein (HP, 150% of SP, n = 7) diet during pregnancy. Offspring (one male and one female per group, n = 14) on day 70 of the embryonic stage and on days 1, 35 and 180 after birth were selected, weighed and killed. Serum samples were analyzed for Tch, insulin and insulin-like growth factor-binding protein 3 (IGFBP-3) levels. Liver samples were analyzed for IGFBP-3 and IGF-I mRNA expression by qRT-PCR and for IGFBP-3, IGF1R and growth hormone receptor (GHR) protein expression by Western blotting. The underlying mechanism of IGFBP-3 regulation was determined by methylated DNA immunoprecipitation (MeDIP) and chromatin immunoprecipitation (ChIP).ResultsHigh-protein exposure resulted in significantly higher body and liver weights of piglets, and it increased their serum T3 and T4 levels at birth and/or at weaning. Furthermore, the IGFBP-3 protein content in the liver and serum was significantly reduced in the HP-exposed weaning piglets, whereas at the transcriptional level IGFBP-3 mRNA expression was downregulated in the livers of HP group piglets. Finally, DNA hypermethylation and higher enrichment of the histone repressive marks H3K27me3 and H3K9me3 were observed.ConclusionsTaken together, these results suggest that a maternal high-protein diet during gestation epigenetically reprograms IGFBP-3 gene expression to modulate the hepatic growth axis in weaning piglets.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Näringslära (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Nutrition and Dietetics (hsv//eng)
Nyckelord
- High-protein diet
- Meishan piglets
- DNA methylation
- Histone modification
- Growth axis
- IGFBP-3
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas