Sökning: WFRF:(Lindberg Benjamin) > Identification of p...
Fältnamn | Indikatorer | Metadata |
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000 | 04454naa a2200481 4500 | |
001 | oai:DiVA.org:kth-212186 | |
003 | SwePub | |
008 | 170818s2017 | |||||||||||000 ||eng| | |
009 | oai:slubar.slu.se:90846 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2121862 URI |
024 | 7 | a https://doi.org/10.1038/s41598-017-06377-82 DOI |
024 | 7 | a https://res.slu.se/id/publ/908462 URI |
040 | a (SwePub)kthd (SwePub)slu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Wahlberg, Elisabetu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Affibody AB4 aut0 (Swepub:slu)47352 |
245 | 1 0 | a Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β |
264 | c 2017-07-20 | |
264 | 1 | b Nature Publishing Group,c 2017 |
338 | a print2 rdacarrier | |
500 | a QC 20170818 | |
520 | a Protofibrils of the 42 amino acids long amyloid-β peptide are transient pre-fibrillar intermediates in the process of peptide aggregation into amyloid plaques and are thought to play a critical role in the pathology of Alzheimer's disease. Hence, there is a need for research reagents and potential diagnostic reagents for detection and imaging of such aggregates. Here we describe an in vitro selection of Affibody molecules that bind to protofibrils of Aβ42cc, which is a stable engineered mimic of wild type Aβ42 protofibrils. Several binders were identified that bind Aβ42cc protofibrils with low nanomolar affinities, and which also recognize wild type Aβ42 protofibrils. Dimeric head-to-tail fusion proteins with subnanomolar binding affinities, and very slow dissociation off-rates, were also constructed. A mapping of the chemical properties of the side chains onto the Affibody scaffold surface reveals three distinct adjacent surface areas of positively charged surface, nonpolar surface and a polar surface, which presumably match a corresponding surface epitope on the protofibrils. The results demonstrate that the engineered Aβ42cc is a suitable antigen for directed evolution of affinity reagents with specificity for wild type Aβ42 protofibrils. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
700 | 1 | a Rahman, Mahafuzuru Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)96558 |
700 | 1 | a Lindberg, Hannau KTH,Proteinteknologi4 aut0 (Swepub:kth)u1mwn94r |
700 | 1 | a Gunneriusson, E.4 aut |
700 | 1 | a Schmuck, Benjaminu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)97577 |
700 | 1 | a Lendel, Christoferu Swedish University of Agricultural Sciences,KTH,Tillämpad fysikalisk kemi,Sveriges lantbruksuniversitet, Sverige,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)50320 |
700 | 1 | a Sandgren, Matsu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)49440 |
700 | 1 | a Löfblom, Johnu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1z7cgkl |
700 | 1 | a Ståhl, Stefanu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1svy8i4 |
700 | 1 | a Härd, Torleifu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences4 aut0 (Swepub:slu)50056 |
710 | 2 | a Sveriges lantbruksuniversitetb Institutionen för Molekylära vetenskaper4 org |
710 | 2 | a Sveriges lantbruksuniversitet |
773 | 0 | t Scientific Reportsd : Nature Publishing Groupg 7:1q 7:1x 2045-2322 |
856 | 4 | u https://www.nature.com/articles/s41598-017-06377-8.pdf |
856 | 4 | u https://pub.epsilon.slu.se/id/eprint/14876/contentsx primaryx Raw objectx freey FULLTEXT |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-212186 |
856 | 4 8 | u https://doi.org/10.1038/s41598-017-06377-8 |
856 | 4 8 | u https://res.slu.se/id/publ/90846 |
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