A nanotube/polymer composite biosensing thin-film transistor platform for C-reactive protein detection

• In the present study, a back-gate thin-film transistor (TFT) based on a single-walled carbon nanotubes (SWCNTs)/poly(9,9-dioctylfluorene-co-bithiophene) (F8T2) composite semiconductor is proposed as electronic label-free immunosensor for the detection of the C-reactive protein (CRP). The nano-composite semiconducting ultra-thin film is deposited on the channel area of the TFT and is functionalized with a monoclonal anti-CRP antibody coating acting as biorecognition layer. The immunesensor was then used for the selective detection of the CRP both in phosphate buffered saline (PBS) solution and in human serum samples. The proposed immunosensor exhibits high current (10 - 20 μA) and good field-effect carriers mobility. Furthermore, the CRP could be revealed at a detection limit as low as 1 mg/L and in a wide dynamic range (from 0.4 nM to 2.2 μM).

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

C-reactive protein detection

label free immunosensor

polymer nano-composite

thin-film transistors

Carbon

Chemical detection

Immunosensors

Nanocomposites

Nanotechnology

Nanotubes

Proteins

Semiconducting organic compounds

Single-walled carbon nanotubes (SWCN)

Thin film transistors

Thin films

Transistors

Ultrathin films

Yarn

C-reactive proteins

Electronic labels

Label-free immunosensor

Phosphate buffered saline solutions

Polymer nanocomposite

Selective detection

Single-walled carbon nanotube (SWCNTs)

Wide dynamic range

Nanocomposite films

Publication and Content Type

ref (subject category)

kon (subject category)