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Deep learning-enhan...
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Wagner, NilsEuropean Molecular Biology Laboratory Heidelberg
(author)
Deep learning-enhanced light-field imaging with continuous validation
- Article/chapterEnglish2021
Publisher, publication year, extent ...
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2021-05-07
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Springer Science and Business Media LLC,2021
Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:24dfdeda-5006-482b-ba55-0d120dd89f7c
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https://lup.lub.lu.se/record/24dfdeda-5006-482b-ba55-0d120dd89f7cURI
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https://doi.org/10.1038/s41592-021-01136-0DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Visualizing dynamic processes over large, three-dimensional fields of view at high speed is essential for many applications in the life sciences. Light-field microscopy (LFM) has emerged as a tool for fast volumetric image acquisition, but its effective throughput and widespread use in biology has been hampered by a computationally demanding and artifact-prone image reconstruction process. Here, we present a framework for artificial intelligence-enhanced microscopy, integrating a hybrid light-field light-sheet microscope and deep learning-based volume reconstruction. In our approach, concomitantly acquired, high-resolution two-dimensional light-sheet images continuously serve as training data and validation for the convolutional neural network reconstructing the raw LFM data during extended volumetric time-lapse imaging experiments. Our network delivers high-quality three-dimensional reconstructions at video-rate throughput, which can be further refined based on the high-resolution light-sheet images. We demonstrate the capabilities of our approach by imaging medaka heart dynamics and zebrafish neural activity with volumetric imaging rates up to 100 Hz.
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Beuttenmueller, FynnHeidelberg University
(author)
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Norlin, NilsLund University,Lunds universitet,Molekylär neuromodulering,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Medicinska fakulteten,Molecular Neuromodulation,Lund University Research Groups,Faculty of Medicine,European Molecular Biology Laboratory Heidelberg(Swepub:lu)ni5154no
(author)
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Gierten, JakobUniversity Hospital Heidelberg
(author)
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Boffi, Juan Carlos
(author)
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Wittbrodt, JoachimHeidelberg University
(author)
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Weigert, MartinSwiss Federal Institute of Technology
(author)
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Hufnagel, Lars
(author)
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Prevedel, RobertEuropean Molecular Biology Laboratory (EMBL Rome)
(author)
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Kreshuk, Anna
(author)
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European Molecular Biology Laboratory HeidelbergHeidelberg University
(creator_code:org_t)
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In:Nature Methods: Springer Science and Business Media LLC18:5, s. 557-5631548-71051548-7091
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Wagner, Nils
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Beuttenmueller, ...
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Norlin, Nils
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Gierten, Jakob
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Boffi, Juan Carl ...
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Wittbrodt, Joach ...
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Weigert, Martin
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Hufnagel, Lars
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Prevedel, Robert
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Kreshuk, Anna
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Radiology Nuclea ...
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Nature Methods
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Lund University