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A new variant 15; 1...
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Axelson, HKarolinska Institutet,Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine
(författare)
A new variant 15; 16 translocation in mouse plasmacytoma leads to the juxtaposition of c-myc and immunoglobulin lambda
- Artikel/kapitelEngelska1991
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:bc3859ad-b4ad-4b85-ab2d-a082fd5c9184
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https://lup.lub.lu.se/record/bc3859ad-b4ad-4b85-ab2d-a082fd5c9184URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Mouse plasmacytomas (MPCs) induced by pristane oil, or by a combination of pristane oil and Abelson virus, carry one of two chromosomal translocations. The typical 12; 15 translocation leads to the juxtaposition of c-myc and immunoglobulin heavy-chain sequences, whereas the 6; 15 translocation links the kappa light-chain locus with the pvt-1 (plasmacytoma variant translocation) locus, located at least 75kb 3' of c-myc [Cory, S., Graham, M., Webb, E., Corcoran, L. & Adams, J. (1985). EMBO J., 4, 675-681]. Unlike the human Burkitt's lymphoma-associated translocation, the lambda/myc juxtaposed variant translocation has not been found previously in MPCs. Using unconventional MPC induction systems in which the tumor precursor cell was induced to proliferate in a secondary host, we have recently identified a 15; 16 translocation in six of the derived MPCs [Wiener, F., Silva, S., Sugiyama, H., Babonits, M. & Klein, G. (1990). Genes Chromosomes Cancer, 2, 36-43]. Chromosome 16 harbors the lambda light-chain gene. To explore whether the 15; 16 translocation represents the lambda/myc juxtaposition, we have mapped the breakpoints on chromosomes 15 and 16 by pulsed-field gel electrophoresis (PFGE). The pvt-1 region was mapped to approximately 220 kb 3' of c-myc. The breakpoint on chromosome 15 in ABPC-Ch-163-10, one of the six 15; 16 translocation-carrying MPCs, was situated approximately 80 kb 3' of c-myc and 140 kb 5' of pvt-1b, the major breakpoint cluster region of the previously analysed 6; 15 variant MPCs. The breakpoint on chromosome 16 was found to cut between the V1 and C3 regions of the lambda locus. Co-migration experiments showed that the C3 and the myc gene were juxtaposed head to tail on the 15; 16 translocation chromosome. On the reciprocal product V1 was juxtaposed to pvt-1.
Ämnesord och genrebeteckningar
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Animals
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Blotting, Northern
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Blotting, Southern
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Chimera
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Chromosome Mapping
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DNA, Neoplasm
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Genes, Immunoglobulin
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Genes, myc
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Immunoglobulin lambda-Chains
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Mice
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Mice, Inbred DBA
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Plasmacytoma
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RNA, Neoplasm
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Restriction Mapping
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Translocation, Genetic
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Panda, C K
(författare)
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Silva, S
(författare)
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Sugiyama, H
(författare)
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Wiener, F
(författare)
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Klein, G
(författare)
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Sumegi, J
(författare)
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Avdelningen för translationell cancerforskningInstitutionen för laboratoriemedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Oncogene6:12, s. 70-22630950-9232
Internetlänk
Hitta via bibliotek
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Oncogene
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