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3D Printed Conducti...
3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
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Bordoni, Matteo (author)
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- Karabulut, Erdem, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kuzmenko, Volodymyr, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fantini, Valentina (author)
- Universita degli studi di Pavia
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Pansarasa, Orietta (author)
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Cereda, Cristina (author)
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- Gatenholm, Paul, 1956 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2020-03-11
- 2020
- English.
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In: Cells. - : MDPI AG. - 2073-4409. ; 9:3
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https://www.mdpi.com...
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https://doi.org/10.3...
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Abstract
Subject headings
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- We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium-induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- carbon nanotubes
- 3D bioprinting
- 3D cell cultures
- cellular models
- conductive scaffold
Publication and Content Type
- art (subject category)
- ref (subject category)
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