High-performance liquid affinity chromatography on silica-bound concanavalin A

• Concanavalin A was covalently bound to porous silica (100-1000 Å) by three different coupling procedures. The performance of the preparations was evaluated from their ability to resolve a mixture of closely related sugars. The Concanavalin A-silica was used for efficient and rapid (5-20 min) analysis/purification of samples containing the glucoproteins peroxidase and glucose oxidase. Such columns could be used at least 60 times with retained performance.

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Publication and Content Type

art (subject category)

ref (subject category)