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Hirudin versus hepa...
Hirudin versus heparin for use in whole blood in vitro biocompatibility models
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- Bexborn, Fredrik (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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- Engberg, Anna E., 1982- (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen,Kristina Nilsson Ekdahl
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- Sandholm, Kerstin (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
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Mollnes, Tom Eirik (författare)
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- Hong, Jaan (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Nilsson Ekdahl, Kristina (författare)
- Uppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Enheten för klinisk immunologi
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(creator_code:org_t)
- Hoboken, NJ, US : John Wiley & Sons Inc, 2009
- 2009
- Engelska.
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Ingår i: Journal of Biomedical Materials Research. Part A. - Hoboken, NJ, US : John Wiley & Sons Inc. - 1549-3296 .- 1552-4965. ; 89A:4, s. 951-959
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Heparin has traditionally been a widely used anticoagulant in blood research, but has been shown to be inappropriate for work with the complement system because of its complement-interacting properties. In this work, we have compared the effects of heparin with those of the specific thrombin inhibitor hirudin on complement and blood cells in vitro. Methods: Whole blood collected in the presence of hirudin (50 µg/mL) or heparin (1 IU/mL) was incubated in the slide chamber model. The plasma was analyzed for complement activation markers C3a and sC5b-9, and the polyvinylchloride test slides were stained for adhering cells. The integrity of the complement system was tested by incubating serum and hirudin-treated plasma in the presence of various activating agents.Results: In contrast to heparin, the addition of hirudin generally preserved the complement reactivity, and complement activation in hirudin plasma closely resembled that in normal serum. Importantly, immunochemical staining of surface-bound cells demonstrated the inducible expression of tissue factor on bound monocytes from hirudin-treated blood, an effect that was completely abolished in heparin-treated blood.Conclusion: Our results indicate that hirudin as an anticoagulant produces more physiological conditions than heparin, making hirudin well-suited for in vitro studies, especially those addressing the regulation of cellular processes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- biocompatibility
- complement
- heparin
- hirudin
- whole blood models
- Immunology
- Immunologi
- Biomedicinsk vetenskap
- Biomedical Sciences
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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