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Genetics and Growth...
Genetics and Growth Regulation in Salmonella enterica
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- Bergman, Jessica M. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Diarmaid Hughes
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- Hughes, Diarmaid, Professor (thesis advisor)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Andersson, Dan I., Professor (thesis advisor)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Ehrenberg, Måns, Professor (thesis advisor)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Maloy, Stanley, Professor (opponent)
- San Diego State University, USA
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(creator_code:org_t)
- ISBN 9789155491000
- Uppsala : Acta Universitatis Upsaliensis, 2014
- English 59 s.
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Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 ; 1052
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Abstract
Subject headings
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- Most free-living bacteria will encounter different environments and it is therefore critical to be able to rapidly adjust to new growth conditions in order to be competitively successful. Responding to changes requires efficient gene regulation in terms of transcription, RNA stability, translation and post-translational modifications.Studies of an extremely slow-growing mutant of Salmonella enterica, with a Glu125Arg mutant version of EF-Tu, revealed it to be trapped in a stringent response. The perceived starvation was demonstrated to be the result of increased mRNA cleavage of aminoacyl-tRNA synthetase genes leading to lower prolyl-tRNA levels. The mutant EF-Tu caused an uncoupling of transcription and translation, leading to increased turnover of mRNA, which trapped the mutant in a futile stringent response.To examine the essentiality of RNase E, we selected and mapped three classes of extragenic suppressors of a ts RNase E phenotype. The ts RNase E mutants were defective in the degradation of mRNA and in the processing of tRNA and rRNA. Only the degradation of mRNA was suppressed by the compensatory mutations. We therefore suggest that degradation of at least a subset of cellular mRNAs is an essential function of RNase E.Bioinformatically, we discovered that the mRNA of tufB, one of the two genes encoding EF-Tu, could form a stable structure masking the ribosomal binding site. This, together with previous studies that suggested that the level of EF-Tu protein could affect the expression of tufB, led us to propose three models for how this could occur. The stability of the tufB RNA structure could be affected by the elongation rate of tufB-translating ribosomes, possibly influenced by the presence of rare codons early in the in tufB mRNA.Using proteomic and genetic assays we concluded that two previously isolated RNAP mutants, each with a growth advantage when present as subpopulations on aging wild-type colonies, were dependent on the utilization of acetate for this phenotype. Increased growth of a subpopulation of wild-type cells on a colony unable to re-assimilate acetate demonstrated that in aging colonies, acetate is available in levels sufficient to sustain the growth of at least a small subpopulation of bacteria.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- tufA
- tufB
- EF-Tu
- ppGpp
- Stringent response
- RNase E
- RNA turnover
- Post-transcriptional regulation
- rpoB
- rpoS
- Growth in stationary phase
- Biology with specialization in Microbiology
- Biologi med inriktning mot mikrobiologi
- Mikrobiologi
- Microbiology
- Molekylär genetik
- Molecular Genetics
- Biology with specialization in Molecular Evolution
- Biologi med inriktning mot molekylär evolution
Publication and Content Type
- vet (subject category)
- dok (subject category)
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