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Verification of mul...
Verification of multi-structure targeting in chronic microelectrode brain recordings from CT scans
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- Censoni, Luciano (författare)
- Umeå University,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Halje, Pär (författare)
- Lund University,Lunds universitet,Integrativ neurofysiologi,Forskargrupper vid Lunds universitet,Neuronano Research Center (NRC),Integrative Neurophysiology,Lund University Research Groups
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- Axelsson, Jan, 1966- (författare)
- Umeå University,Umeå universitet,Radiofysik
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- Skovgård, Katrine (författare)
- Lund University,Lunds universitet,Basala gangliernas patofysiologi,Forskargrupper vid Lunds universitet,Basal Ganglia Pathophysiology,Lund University Research Groups
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- Ramezani, Arash (författare)
- Umeå University,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Malinina, Evgenya (författare)
- Umeå University,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Petersson, Per (författare)
- Umeå University,Lund University,Lunds universitet,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),The Group for Integrative Neurophysiology and Neurotechnology, Department of Experimental Medical Science, Lund University, Lund, Sweden,Integrativ neurofysiologi,Forskargrupper vid Lunds universitet,Neuronano Research Center (NRC),LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Integrative Neurophysiology,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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(creator_code:org_t)
- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: Journal of Neuroscience Methods. - : Elsevier. - 0165-0270 .- 1872-678X. ; 382
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Background: Large-scale microelectrode recordings offer a unique opportunity to study neurophysiological processes at the network level with single cell resolution. However, in the small brains of many experimental animals, it is often technically challenging to verify the correct targeting of the intended structures, which inherently limits the reproducibility of acquired data.New method: To mitigate this problem, we have developed a method to programmatically segment the trajectory of electrodes arranged in larger arrays from acquired CT-images and thereby determine the position of individual recording tips with high spatial resolution, while also allowing for coregistration with an anatomical atlas, without pre-processing of the animal samples or post-imaging histological analyses.Results: Testing the technical limitations of the developed method, we found that the choice of scanning angle influences the achievable spatial resolution due to shadowing effects caused by the electrodes. However, under optimal acquisition conditions, individual electrode tip locations within arrays with 250 µm inter-electrode spacing were possible to reliably determine.Comparison to existing methods: Comparison to a histological verification method suggested that, under conditions where individual wires are possible to track in slices, a 90% correspondence could be achieved in terms of the number of electrodes groups that could be reliably assigned to the same anatomical structure.Conclusions: The herein reported semi-automated procedure to verify anatomical targeting of brain structures in the rodent brain could help increasing the quality and reproducibility of acquired neurophysiological data by reducing the risk of assigning recorded brain activity to incorrectly identified anatomical locations.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
Nyckelord
- Imaging
- Microelectrode
- Mouse
- Neurophysiology
- Rat
- X-ray
- Neurology
- neurologi
- Imaging
- Microelectrode
- Mouse
- Neurophysiology
- Rat
- X-ray
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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