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Fast exocytosis wit...
Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells
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- Barg, Sebastian (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Ma, Xiaosong (författare)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Eliasson, Lena (författare)
- Lund University,Lunds universitet,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Galvanovskis, Juris (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Göpel, Sven (författare)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Obermüller, Stefanie (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Platzer, Josef (författare)
- Institut für Biochemische Pharmakologie, Innsbruck
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- Renström, Erik (författare)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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- Trus, Michel (författare)
- Department of Biological Chemistry, The Hebrew University of Jerusalem
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- Atlas, Daphne (författare)
- Department of Biological Chemistry, The Hebrew University of Jerusalem
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- Striessnig, Jörg (författare)
- Institut für Biochemische Pharmakologie, Innsbruck
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- Rorsman, Patrik (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Molecular and Cellular Physiology, Institute of Physiological Sciences, Lund University, Lund
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Biophysical Journal. - 1542-0086 .- 0006-3495. ; 81:6, s. 3308-3323
- Relaterad länk:
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http://www.biophysj....
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https://lup.lub.lu.s...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The association of L-type Ca(2+) channels to the secretory granules and its functional significance to secretion was investigated in mouse pancreatic B cells. Nonstationary fluctuation analysis showed that the B cell is equipped with <500 alpha1(C) L-type Ca(2+) channels, corresponding to a Ca(2+) channel density of 0.9 channels per microm(2). Analysis of the kinetics of exocytosis during voltage-clamp depolarizations revealed an early component that reached a peak rate of 1.1 pFs(-1) (approximately 650 granules/s) 25 ms after onset of the pulse and is completed within approximately 100 ms. This component represents a subset of approximately 60 granules situated in the immediate vicinity of the L-type Ca(2+) channels, corresponding to approximately 10% of the readily releasable pool of granules. Experiments involving photorelease of caged Ca(2+) revealed that the rate of exocytosis was half-maximal at a cytoplasmic Ca(2+) concentration of 17 microM, and concentrations >25 microM are required to attain the rate of exocytosis observed during voltage-clamp depolarizations. The rapid component of exocytosis was not affected by inclusion of millimolar concentrations of the Ca(2+) buffer EGTA but abolished by addition of exogenous L(C753-893), the 140 amino acids of the cytoplasmic loop connecting the 2(nd) and 3(rd) transmembrane region of the alpha1(C) L-type Ca(2+) channel, which has been proposed to tether the Ca(2+) channels to the secretory granules. In keeping with the idea that secretion is determined by Ca(2+) influx through individual Ca(2+) channels, exocytosis triggered by brief (15 ms) depolarizations was enhanced 2.5-fold by the Ca(2+) channel agonist BayK8644 and 3.5-fold by elevating extracellular Ca(2+) from 2.6 to 10 mM. Recordings of single Ca(2+) channel activity revealed that patches predominantly contained no channels or many active channels. We propose that several Ca(2+) channels associate with a single granule thus forming a functional unit. This arrangement is important in a cell with few Ca(2+) channels as it ensures maximum usage of the Ca(2+) entering the cell while minimizing the influence of stochastic variations of the Ca(2+) channel activity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
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Barg, Sebastian
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Ma, Xiaosong
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Eliasson, Lena
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Galvanovskis, Ju ...
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Göpel, Sven
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Obermüller, Stef ...
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visa fler...
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Platzer, Josef
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Renström, Erik
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Trus, Michel
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Atlas, Daphne
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Striessnig, Jörg
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Rorsman, Patrik
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