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Clathrin coat contr...
Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity
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- Farsi, Zohreh (author)
- Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany.;Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany.
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- Gowrisankaran, Sindhuja (author)
- Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.
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- Krunic, Matija (author)
- Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.
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- Rammner, Burkhard (author)
- Sciloop, Hamburg, Germany.
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- Woehler, Andrew (author)
- Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany.
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- Lafer, Eileen M. (author)
- Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem & Struct Biol, San Antonio, TX 78229 USA.;Univ Texas Hlth Sci Ctr San Antonio, Ctr Biomed Neurosci, San Antonio, TX 78229 USA.
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- Mim, Carsten (author)
- KTH,Medicinteknik och hälsosystem
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- Jahn, Reinhard (author)
- Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany.
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- Milosevic, Ira (author)
- Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany.
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Max Planck Inst Biophys Chem, Dept Neurobiol, Gottingen, Germany;Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany. Univ Med Ctr Gottingen, European Neurosci Inst, Synapt Vesicle Dynam Grp, Gottingen, Germany. (creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2018
- 2018
- English.
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In: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 7
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.7...
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Abstract
Subject headings
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- Newly-formed synaptic vesicles (SVs) are rapidly acidified by vacuolar adenosine triphosphatases (vATPases), generating a proton electrochemical gradient that drives neurotransmitter loading. Clathrin-mediated endocytosis is needed for the formation of new SVs, yet it is unclear when endocytosed vesicles acidify and refill at the synapse. Here, we isolated clathrin-coated vesicles (CCVs) from mouse brain to measure their acidification directly at the single vesicle level. We observed that the ATP-induced acidification of CCVs was strikingly reduced in comparison to SVs. Remarkably, when the coat was removed from CCVs, uncoated vesicles regained ATP-dependent acidification, demonstrating that CCVs contain the functional vATPase, yet its function is inhibited by the clathrin coat. Considering the known structures of the vATPase and clathrin coat, we propose a model in which the formation of the coat surrounds the vATPase and blocks its activity. Such inhibition is likely fundamental for the proper timing of SV refilling.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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