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Playing Peekaboo wi...
Playing Peekaboo with a Master Manipulator: Metagenetic Detection and Phylogenetic Analysis of Wolbachia Supergroups in Freshwater Invertebrates
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- Mioduchowska, Monika (författare)
- Univ Gdansk, Poland; Univ Lodz, Poland; Univ Gdansk, Poland
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- Konecka, Edyta (författare)
- Adam Mickiewicz Univ, Poland
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- Goldyn, Bartlomiej (författare)
- Adam Mickiewicz Univ, Poland
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- Pinceel, Tom (författare)
- Katholieke Univ Leuven, Belgium; Univ Free State, South Africa; Vrije Univ Brussel VUB, Belgium
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- Brendonck, Luc (författare)
- Katholieke Univ Leuven, Belgium; North West Univ, South Africa
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- Lukic, Dunja (författare)
- CSIC, Spain
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- Kaczmarek, Lukasz (författare)
- Adam Mickiewicz Univ, Poland
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- Namiotko, Tadeusz (författare)
- Univ Gdansk, Poland
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- Zajac, Katarzyna (författare)
- Polish Acad Sci, Poland
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- Zajac, Tadeusz (författare)
- Polish Acad Sci, Poland
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- Jastrzebski, Jan P. (författare)
- Univ Warm & Mazury Olsztyn, Poland; Univ Warm & Mazury Olsztyn, Poland
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- Bartoszek, Krzysztof (författare)
- Linköpings universitet,Statistik och maskininlärning,Filosofiska fakulteten
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(creator_code:org_t)
- MDPI, 2023
- 2023
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 24:11
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- The infamous "master manipulators"-intracellular bacteria of the genus Wolbachia-infect a broad range of phylogenetically diverse invertebrate hosts in terrestrial ecosystems. Wolbachia has an important impact on the ecology and evolution of their host with documented effects including induced parthenogenesis, male killing, feminization, and cytoplasmic incompatibility. Nonetheless, data on Wolbachia infections in non-terrestrial invertebrates are scarce. Sampling bias and methodological limitations are some of the reasons limiting the detection of these bacteria in aquatic organisms. In this study, we present a new metagenetic method for detecting the co-occurrence of different Wolbachia strains in freshwater invertebrates host species, i.e., freshwater Arthropoda (Crustacea), Mollusca (Bivalvia), and water bears (Tardigrada) by applying NGS primers designed by us and a Python script that allows the identification of Wolbachia target sequences from the microbiome communities. We also compare the results obtained using the commonly applied NGS primers and the Sanger sequencing approach. Finally, we describe three supergroups of Wolbachia: (i) a new supergroup V identified in Crustacea and Bivalvia hosts; (ii) supergroup A identified in Crustacea, Bivalvia, and Eutardigrada hosts, and (iii) supergroup E infection in the Crustacea host microbiome community.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Crustacea; Bivalvia; Tardigrada; next-generation sequencing; microbiome communities; Wolbachia endosymbiont
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Mioduchowska, Mo ...
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Konecka, Edyta
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Goldyn, Bartlomi ...
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Pinceel, Tom
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Brendonck, Luc
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Lukic, Dunja
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Kaczmarek, Lukas ...
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Namiotko, Tadeus ...
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Zajac, Katarzyna
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Zajac, Tadeusz
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Jastrzebski, Jan ...
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Bartoszek, Krzys ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Odontologi
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International Jo ...
- Av lärosätet
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Linköpings universitet