WFRF:(Ke Rongqin)
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In situ sequencing for RNA analysis in preserved tissue and cells
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- Ke, Rongqin (author)
- Stockholms universitet,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Uppsala University
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- Mignardi, Marco (author)
- Stockholms universitet,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Uppsala University
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- Pacureanu, Alexandra (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Avdelningen för visuell information och interaktion,Bildanalys och människa-datorinteraktion
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- (creator_code:org_t)
- 2013-07-14
- 2013
- English.
- In: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 10:9, s. 857-860
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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