Three exceptional IgH/myc-translocation-carrying rat immunocytomas have breakpoints 50 to 80 kb 5' of c-myc

• The spontaneously arising immunocytoma of the Louvain rat (RIC) carries a consistent chromosomal translocation between chromosomes 6 and 7. This translocation juxtaposes immunoglobulin heavy chain and c-myc sequences. In an earlier study on 14 RIC tumors, we found that the translocation breakpoint is located within 1.5 kb immediately upstream of c-myc in 10 of the tumors. Here we describe 3 exceptional tumors that had no rearrangement within 20 kb 5' of c-myc. Using pulsed-field gel electrophoresis we show that the translocation breakpoints in these tumors are located 50-80 kb 5' of c-myc and that c-myc rearranges to the 3' end of the IgH cluster.

Nyckelord

Animals

Cell Line

Chromosome Mapping

DNA, Neoplasm

Genes, Immunoglobulin

Genes, myc

Immunoglobulin Heavy Chains

Leukemia, Lymphocytic, Chronic, B-Cell

Multigene Family

Rats

Rats, Inbred Strains

Restriction Mapping

Translocation, Genetic

Tumor Cells, Cultured

Publikations- och innehållstyp

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