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Bromodeoxyuridine a...
Bromodeoxyuridine and the detection of neurogenesis.
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- Kuhn, Hans-Georg, 1961 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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- Cooper-Kuhn, Christiana M, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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(creator_code:org_t)
- 2007
- 2007
- Engelska.
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Ingår i: Current pharmaceutical biotechnology. - 1873-4316. ; 8:3, s. 127-31
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Bromodeoxyuridine (BrdU) is widely used for labeling dividing cells to determine their fate. In particular, the analysis of neurogenesis in the adult mammalian brain has made significant progress through the use of this technique. However; when using BrdU for labeling, there are several issues to consider in order to minimalize possible cytotoxicity or false-positive labeling. This current review summarizes methodological and technical aspects of BrdU administration and detection, compares alternative methods and gives recommendations on how to avoid labeling artifacts.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Animals
- Artifacts
- Bromodeoxyuridine
- analysis
- Cell Proliferation
- Cells
- Cultured
- Humans
- Image Enhancement
- methods
- Microscopy
- Fluorescence
- methods
- Nerve Regeneration
- physiology
- Neurons
- cytology
- drug effects
- physiology
- Staining and Labeling
- methods
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
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