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ZBED6, a novel tran...
ZBED6, a novel transcription factor derived from a domesticated DNA transposon regulates IGF2 expression and muscle growth
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- Markljung, Ellen (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Jiang, Lin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Jaffe, Jacob D. (author)
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show more...
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Mikkelsen, Tarjei S. (author)
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- Wallerman, Ola (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Larhammar, Martin (author)
- Uppsala universitet,Genetisk utvecklingsbiologi
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Zhang, Xiaolan (author)
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Wang, Li (author)
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Saenz-Vash, Veronica (author)
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Gnirke, Andreas (author)
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- Lindroth, Anders (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics
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Barrés, Romain (author)
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- Yan, Jie (author)
- Karolinska Institutet
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- Strömberg, Sara (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- De, Sachinandan (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Pontén, Fredrik (author)
- Uppsala universitet,Institutionen för genetik och patologi
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Lander, Eric S. (author)
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Carr, Steven A. (author)
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- Zierath, Juleen R. (author)
- Karolinska Institutet
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- Kullander, Klas (author)
- Uppsala universitet,Genetisk utvecklingsbiologi
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- Wadelius, Claes (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Lindblad-Toh, Kerstin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Andersson, Göran (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics
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- Hjälm, Göran (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Andersson, Leif (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics,Uppsala University
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(creator_code:org_t)
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- 2009-12-15
- 2009
- English.
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In: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 7:12, s. e1000256-
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Subject headings
Close
- A single nucleotide substitution in intron 3 of IGF2 in pigs abrogates a binding site for a repressor and leads to a 3-fold up-regulation of IGF2 in skeletal muscle. The mutation has major effects on muscle growth, size of the heart, and fat deposition. Here, we have identified the repressor and find that the protein, named ZBED6, is previously unknown, specific for placental mammals, and derived from an exapted DNA transposon. Silencing of Zbed6 in mouse C2C12 myoblasts affected Igf2 expression, cell proliferation, wound healing, and myotube formation. Chromatin immunoprecipitation (ChIP) sequencing using C2C12 cells identified about 2,500 ZBED6 binding sites in the genome, and the deduced consensus motif gave a perfect match with the established binding site in Igf2. Genes associated with ZBED6 binding sites showed a highly significant enrichment for certain Gene Ontology classifications, including development and transcriptional regulation. The phenotypic effects in mutant pigs and ZBED6-silenced C2C12 myoblasts, the extreme sequence conservation, its nucleolar localization, the broad tissue distribution, and the many target genes with essential biological functions suggest that ZBED6 is an important transcription factor in placental mammals, affecting development, cell proliferation, and growth.
Subject headings
- LANTBRUKSVETENSKAPER -- Veterinärmedicin (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science (hsv//eng)
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Markljung, Ellen
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Jiang, Lin
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Jaffe, Jacob D.
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Mikkelsen, Tarje ...
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Wallerman, Ola
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Larhammar, Marti ...
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show more...
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Zhang, Xiaolan
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Wang, Li
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Saenz-Vash, Vero ...
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Gnirke, Andreas
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Lindroth, Anders
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Barrés, Romain
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Yan, Jie
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Strömberg, Sara
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De, Sachinandan
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Pontén, Fredrik
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Lander, Eric S.
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Carr, Steven A.
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Zierath, Juleen ...
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Kullander, Klas
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Wadelius, Claes
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Lindblad-Toh, Ke ...
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Andersson, Göran
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Hjälm, Göran
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Andersson, Leif
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show less...
- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Veterinary Scien ...
- Articles in the publication
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PLoS biology
- By the university
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Uppsala University
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Karolinska Institutet
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Swedish University of Agricultural Sciences