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Imaging dynamics of...
Imaging dynamics of CD11c(+) cells and Foxp3(+) cells in progressive autoimmune insulitis in the NOD mouse model of type 1 diabetes
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- Schmidt-Christensen, Anja (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Hansen, Lisbeth (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Ilegems, Erwin (författare)
- Karolinska Institutet
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- Fransén Pettersson, Nina (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Dahl, Ulf (författare)
- Umeå universitet,Klinisk immunologi
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- Gupta, Shashank (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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- Larefalk, Åsa (författare)
- Umeå universitet,Klinisk immunologi
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- Hannibal, Tine (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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Schulz, Alexander (författare)
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- Berggren, Per-Olof (författare)
- Karolinska Institutet
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- Holmberg, Dan (författare)
- Lund University,Lunds universitet,Autoimmunitet,Forskargrupper vid Lunds universitet,Autoimmunity,Lund University Research Groups
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(creator_code:org_t)
- 2013-08-21
- 2013
- Engelska.
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Ingår i: Diabetologia. - : Springer Science and Business Media LLC. - 1432-0428 .- 0012-186X. ; 56:12, s. 2669-2678
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The aim of this study was to visualise the dynamics and interactions of the cells involved in autoimmune-driven inflammation in type 1 diabetes. We adopted the anterior chamber of the eye (ACE) transplantation model to perform non-invasive imaging of leucocytes infiltrating the endocrine pancreas during initiation and progression of insulitis in the NOD mouse. Individual, ACE-transplanted islets of Langerhans were longitudinally and repetitively imaged by stereomicroscopy and two-photon microscopy to follow fluorescently labelled leucocyte subsets. We demonstrate that, in spite of the immune privileged status of the eye, the ACE-transplanted islets develop infiltration and beta cell destruction, recapitulating the autoimmune insulitis of the pancreas, and exemplify this by analysing reporter cell populations expressing green fluorescent protein under the Cd11c or Foxp3 promoters. We also provide evidence that differences in morphological appearance of subpopulations of infiltrating leucocytes can be correlated to their distinct dynamic behaviour. Together, these findings demonstrate that the kinetics and dynamics of these key cellular components of autoimmune diabetes can be elucidated using this imaging platform for single cell resolution, non-invasive and repetitive monitoring of the individual islets of Langerhans during the natural development of autoimmune diabetes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Animal-mouse
- Imaging
- Islet degeneration and damage
- Islet
- transplantation
- Animal-mouse
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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