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Controlled Assembly of Gold Nanoparticles using De Novo Designed Polypeptide Scaffolds

Aili, Daniel, 1977- (author)
Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
Enander, Karin (author)
Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
Rydberg, Johan (author)
Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
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Nesterenko, Irina (author)
Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan
Björefors, Fredrik (author)
Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
Baltzer, Lars (author)
Division of Organic Chemistry, Department of Biochemistry and Organic Chemistry, BMC, Box 599, Uppsala University, SE-751 24 Uppsala, Sweden.
Liedberg, Bo (author)
Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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 (creator_code:org_t)
SPIE, 2008
2008
English.
In: Proceedings SPIE, Vol. 6885, Photonic Biosensing and Microoptics. - : SPIE. ; , s. 688506-1-688506-8
  • Conference paper (peer-reviewed)
Abstract Subject headings
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  • Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides and gold nanoparticles, particle-peptide hybrid multi layers could be formed.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Other Basic Medicine (hsv//eng)

Keyword

Heterodimerization
polypeptides
gold nanoparticles
four-helix bundle
helix-loop-helix
self-assembly
Chemistry
Kemi

Publication and Content Type

ref (subject category)
kon (subject category)

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Aili, Daniel, 19 ...
Enander, Karin
Rydberg, Johan
Nesterenko, Irin ...
Björefors, Fredr ...
Baltzer, Lars
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Liedberg, Bo
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About the subject
MEDICAL AND HEALTH SCIENCES
MEDICAL AND HEAL ...
and Basic Medicine
and Other Basic Medi ...
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Linköping University

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