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Divergent Transcrip...
Divergent Transcriptional Responses to Physiological and Xenobiotic Stress in Giardia duodenalis
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- Ansell, Brendan R. E. (författare)
- Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia.
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- McConville, Malcolm J. (författare)
- Univ Melbourne, Bio21 Mol Sci & Biotechnol Inst, Melbourne, Vic, Australia.
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- Baker, Louise (författare)
- Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia.;Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun, Melbourne, Vic, Australia.
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- Korhonen, Pasi K. (författare)
- Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia.
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- Emery, Samantha J. (författare)
- Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun, Melbourne, Vic, Australia.
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- Svärd, Staffan G (författare)
- Uppsala universitet,Mikrobiologi
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- Gasser, Robin B. (författare)
- Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia.
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- Jex, Aaron R. (författare)
- Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia.;Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun, Melbourne, Vic, Australia.
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Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia Univ Melbourne, Bio21 Mol Sci & Biotechnol Inst, Melbourne, Vic, Australia. (creator_code:org_t)
- 2016
- 2016
- Engelska.
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Ingår i: Antimicrobial Agents and Chemotherapy. - 0066-4804 .- 1098-6596. ; 60:10, s. 6034-6045
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Understanding how parasites respond to stress can help to identify essential biological processes. Giardia duodenalis is a parasitic protist that infects the human gastrointestinal tract and causes 200 to 300 million cases of diarrhea annually. Metronidazole, a major antigiardial drug, is thought to cause oxidative damage within the infective trophozoite form. However, treatment efficacy is suboptimal, due partly to metronidazole-resistant infections. To elucidate conserved and stress-specific responses, we calibrated sublethal metronidazole, hydrogen peroxide, and thermal stresses to exert approximately equal pressure on trophozoite growth and compared transcriptional responses after 24 h of exposure. We identified 252 genes that were differentially transcribed in response to all three stressors, including glycolytic and DNA repair enzymes, a mitogen-activated protein (MAP) kinase, high-cysteine membrane proteins, flavin adenine dinucleotide (FAD) synthetase, and histone modification enzymes. Transcriptional responses appeared to diverge according to physiological or xenobiotic stress. Downregulation of the antioxidant system and alpha-giardins was observed only under metronidazole-induced stress, whereas upregulation of GARP-like transcription factors and their subordinate genes was observed in response to hydrogen peroxide and thermal stressors. Limited evidence was found in support of stress-specific response elements upstream of differentially transcribed genes; however, antisense derepression and differential regulation of RNA interference machinery suggest multiple epigenetic mechanisms of transcriptional control.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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