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Time-resolved protein nanocrystallography using an X-ray free-electron laser

Aquila, Andrew (author)
Hunter, Mark S. (author)
Doak, R. Bruce (author)
show more...
Kirian, Richard A. (author)
Fromme, Petra (author)
White, Thomas A. (author)
Andreasson, Jakob (author)
Uppsala universitet,Molekylär biofysik
Arnlund, David (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Bajt, Saša (author)
Barends, Thomas R. M. (author)
Barthelmess, Miriam (author)
Bogan, Michael J. (author)
Bostedt, Christoph (author)
Bottin, Hervé (author)
Bozek, John D. (author)
Caleman, Carl (author)
Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Molekylär biofysik
Coppola, Nicola (author)
Davidsson, Jan (author)
Uppsala universitet,Fysikalisk kemi
DePonte, Daniel P. (author)
Elser, Veit (author)
Epp, Sascha W. (author)
Erk, Benjamin (author)
Fleckenstein, Holger (author)
Foucar, Lutz (author)
Frank, Matthias (author)
Fromme, Raimund (author)
Graafsma, Heinz (author)
Grotjohann, Ingo (author)
Gumprecht, Lars (author)
Hajdu, Janos (author)
Uppsala universitet,Molekylär biofysik
Hampton, Christina Y. (author)
Hartmann, Andreas (author)
Hartmann, Robert (author)
Hau-Riege, Stefan (author)
Hauser, Günter (author)
Hirsemann, Helmut (author)
Holl, Peter (author)
Holton, James M. (author)
Hömke, André (author)
Johansson, Linda (author)
Kimmel, Nils (author)
Kassemeyer, Stephan (author)
Krasniqi, Faton (author)
Kühnel, Kai-Uwe (author)
Liang, Mengning (author)
Lomb, Lukas (author)
Malmerberg, Erik, 1980 (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Marchesini, Stefano (author)
Martin, Andrew V. (author)
Maia, Filipe R.N.C. (author)
Gothenburg University,Göteborgs universitet,Uppsala universitet,Molekylär biofysik,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Seibert, M. Marvin (author)
Uppsala universitet,Molekylär biofysik
Nass, Karol (author)
Reich, Christian (author)
Neutze, Richard (author)
Rolles, Daniel (author)
Rudek, Benedikt (author)
Rudenko, Artem (author)
Schlichting, Ilme (author)
Schmidt, Carlo (author)
Schmidt, Kevin E. (author)
Schulz, Joachim (author)
Seibert, M. Marvin (author)
Uppsala universitet,Molekylär biofysik
Soltau, Heike (author)
Shoeman, Robert L. (author)
Sierra, Raymond (author)
Starodub, Dmitri (author)
Stellato, Francesco (author)
Stern, Stephan (author)
Strüder, Lothar (author)
Timneanu, Nicusor (author)
Uppsala universitet,Molekylär biofysik
Ullrich, Joachim (author)
Wang, Xiaoyu (author)
Williams, Garth J. (author)
Weidenspointner, Georg (author)
Weierstall, Uwe (author)
Wunderer, Cornelia (author)
Barty, Anton (author)
Spence, John C. H. (author)
Chapman, Henry N. (author)
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 (creator_code:org_t)
2012
2012
English.
In: Optics Express. - 1094-4087. ; 20:3, s. 2706-2716
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Laser-induced chemistry
Ultrafast lasers
Medical optics and biotechnology
Ultrafast technology
X-ray imaging

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor
Aquila, Andrew
Hunter, Mark S.
Doak, R. Bruce
Kirian, Richard ...
Fromme, Petra
White, Thomas A.
show more...
Andreasson, Jako ...
Arnlund, David
Bajt, Saša
Barends, Thomas ...
Barthelmess, Mir ...
Bogan, Michael J ...
Bostedt, Christo ...
Bottin, Hervé
Bozek, John D.
Caleman, Carl
Coppola, Nicola
Davidsson, Jan
DePonte, Daniel ...
Elser, Veit
Epp, Sascha W.
Erk, Benjamin
Fleckenstein, Ho ...
Foucar, Lutz
Frank, Matthias
Fromme, Raimund
Graafsma, Heinz
Grotjohann, Ingo
Gumprecht, Lars
Hajdu, Janos
Hampton, Christi ...
Hartmann, Andrea ...
Hartmann, Robert
Hau-Riege, Stefa ...
Hauser, Günter
Hirsemann, Helmu ...
Holl, Peter
Holton, James M.
Hömke, André
Johansson, Linda
Kimmel, Nils
Kassemeyer, Step ...
Krasniqi, Faton
Kühnel, Kai-Uwe
Liang, Mengning
Lomb, Lukas
Malmerberg, Erik ...
Marchesini, Stef ...
Martin, Andrew V ...
Maia, Filipe R.N ...
Seibert, M. Marv ...
Nass, Karol
Reich, Christian
Neutze, Richard
Rolles, Daniel
Rudek, Benedikt
Rudenko, Artem
Schlichting, Ilm ...
Schmidt, Carlo
Schmidt, Kevin E ...
Schulz, Joachim
Soltau, Heike
Shoeman, Robert ...
Sierra, Raymond
Starodub, Dmitri
Stellato, France ...
Stern, Stephan
Strüder, Lothar
Timneanu, Nicuso ...
Ullrich, Joachim
Wang, Xiaoyu
Williams, Garth ...
Weidenspointner, ...
Weierstall, Uwe
Wunderer, Cornel ...
Barty, Anton
Spence, John C. ...
Chapman, Henry N ...
show less...
About the subject
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Structural Biolo ...
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Biophysics
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Biochemistry and ...
Articles in the publication
Optics Express
By the university
Uppsala University
University of Gothenburg

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