SwePub
Tyck till om SwePub Sök här!
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:DiVA.org:umu-189495"
 

Sökning: onr:"swepub:oai:DiVA.org:umu-189495" > Multi-laboratory ex...

Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis

Colomé, Núria (författare)
ProteoRed-ISCIII, Vall d'Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain
Abian, Joaquín (författare)
ProteoRed-ISCIII, Instituto de Investigaciones Biomédicas de Barcelona, IIBB-CSIC/IDIBAPS, 08036 Barcelona, Spain
Aloria, Kerman (författare)
ProteoRed-ISCIII, Proteomics Core Facility-SGIKER, University of the Basque Country (UPV/EHU), Leioa, Spain
visa fler...
Arizmendi, Jesús M. (författare)
Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain
Barceló-Batllori, Silvia (författare)
ProteoRed-ISCIII, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
Braga-Lagache, Sophie (författare)
Department for BioMedical Research (DBMR), Proteomics and Mass Spectrometry Core Facility, University of Bern, CH-3010 Bern, Switzerland
Burlet-Schiltz, Odile (författare)
Proteomics and Mass Spectrometry of Biomolecules, Proteomics Infrastructure of Toulouse, Proteomics French Infrastructure, ProFI. Institut de Pharmacologie et Biologie Structurale (IPBS), Université de Toulouse, UPS, CNRS, Toulouse, France
Carrascal, Montse (författare)
ProteoRed-ISCIII, Instituto de Investigaciones Biomédicas de Barcelona, IIBB-CSIC/IDIBAPS, 08036 Barcelona, Spain
Casal, J. Ignacio (författare)
ProteoRed-ISCIII, Centro de Investigaciones Biológicas-CSIC, Madrid 28040, Spain
Chicano-Gálvez, Eduard (författare)
ProteoRed-ISCIII, Proteomics Unit, IMIBIC/UCO/HURS, IMIBIC Building Fl.3, 14004 Córdoba, Spain
Chiva, Cristina (författare)
Proteomics Unit, Center for Genomics Regulation, Barcelona Institute of Science and Technology (BIST), Barcelona, Spain
Clemente, Luis Felipe (författare)
ProteoRed-ISCIII, Proteomics Unit, Complutense University, 28040 Madrid, Spain
Elortza, Felix (författare)
ProteoRed-ISCIII, CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), CIBERehd,Bizkaia Science and Technology Park, 48160 Derio, Spain
Estanyol, Josep M. (författare)
ProteoRed-ISCIII, Scientific and Technological Centers (CCiTUB), University of Barcelona, 08036 Barcelona, Spain
Fernandez-Irigoyen, Joaquín (författare)
Proteored-ISCIII. Proteomics Unit, Clinical Neuroproteomics Group, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, 31008 Pamplona, Spain
Fernández-Puente, Patricia (författare)
Grupo de Investigación de Reumatología (GIR), Agrupación CICA-INIBIC, Universidad de A Coruña, A Coruña, Spain
Fidalgo, María José (författare)
ProteoRed-ISCIII, Scientific and Technological Centers (CCiTUB), University of Barcelona, 08036 Barcelona, Spain
Froment, Carine (författare)
Proteomics and Mass Spectrometry of Biomolecules, Proteomics Infrastructure of Toulouse, Proteomics French Infrastructure, ProFI. Institut de Pharmacologie et Biologie Structurale (IPBS), Université de Toulouse, UPS, CNRS, Toulouse, France
Fuentes, Manuel (författare)
Department of Medicine and General Cytometry Service-Nucleus, Proteomics Unit, CIBERONC, Cancer Research Center (IBMCC/CSIC/USAL/IBSAL), Universidad de Salamanca, Spain
Fuentes-Almagro, Carlos (författare)
Proteomics Unit, SCAI, University of Córdoba, Ramón y Cajal Building, Rabanales Campus, 14071, Córdoba, Spain
Gay, Marina (författare)
ProteoRed-ISCIII, Institute for Research in Biomedicine (IRB Barcelona), BIST (The Barcelona Institute of Science and Technology), Baldiri i Reixac 10, 08028 Barcelona, Spain
Hainard, Alexandre (författare)
Proteomics Core Facility, CMU, University of Geneva, Switzerland
Heller, Manfred (författare)
Department for BioMedical Research (DBMR), Proteomics and Mass Spectrometry Core Facility, University of Bern, CH-3010 Bern, Switzerland
Hernández, María Luisa (författare)
ProteoRed-ISCIII, Proteomics Unit, Complutense University, 28040 Madrid, Spain
Ibarrola, Nieves (författare)
ProteoRed-ISCIII, Proteomics Unit. Cancer Research Center (IBMCC/CSIC/USAL/IBSAL), Universidad de Salamanca-CSIC, Salamanca, Spain
Iloro, Ibon (författare)
ProteoRed-ISCIII, CIC bioGUNE, Proteomics Platform, Basque Research & Technology Alliance (BRTA), CIBERehd,Bizkaia Science and Technology Park, 48160 Derio, Spain
Kieselbach, Thomas (författare)
Umeå universitet,Kemiska institutionen,Thomas Kieselbach
Lario, Antonio (författare)
ProteoRed-ISCIII, IPBLN -CSIC, 18016 Granada, Spain
Locard-Paulet, Marie (författare)
Proteomics and Mass Spectrometry of Biomolecules, Proteomics Infrastructure of Toulouse, Proteomics French Infrastructure, ProFI. Institut de Pharmacologie et Biologie Structurale (IPBS), Université de Toulouse, UPS, CNRS, Toulouse, France
Marina-Ramírez, Anabel (författare)
ProteoRed-ISCIII, CBM Severo Ochoa (CSIC-UAM), Madrid 28049, Spain
Martín, Luna (författare)
ProteoRed-ISCIII, Vall d'Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain
Morato-López, Esperanza (författare)
ProteoRed-ISCIII, CBM Severo Ochoa (CSIC-UAM), Madrid 28049, Spain
Muñoz, Javier (författare)
ProteoRed-ISCIII, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain
Navajas, Rosana (författare)
ProteoRed-ISCIII, Centro Nacional de Biotecnologia (CSIC), 28049, Madrid, Spain
Odena, M. Antonia (författare)
ProteoRed-ISCIII, Proteomics Platform, Barcelona Science Park, 08028, Barcelona, Spain
Odriozola, Leticia (författare)
ProteoRed-ISCIII, CIMA, University of Navarra, 31008, Pamplona, Spain
de Oliveira, Eliandre (författare)
ProteoRed-ISCIII, Proteomics Platform, Barcelona Science Park, 08028, Barcelona, Spain
Paradela, Alberto (författare)
ProteoRed-ISCIII, Centro Nacional de Biotecnologia (CSIC), 28049, Madrid, Spain
Pasquarello, Carla (författare)
Proteomics Core Facility, CMU, University of Geneva, Switzerland
de los Rios, Vivian (författare)
ProteoRed-ISCIII, Centro de Investigaciones Biológicas-CSIC, Madrid 28040, Spain
Ruiz-Romero, Cristina (författare)
Grupo de Investigación de Reumatología (GIR) - ProteoRed-ISCIII, Unidad de Proteómica, INIBIC–Complejo Hospitalario Universitario de A Coruña, SERGAS, A Coruña, Spain
Sabidó, Eduard (författare)
ProteoRed ISCIII, Proteomics Unit, Universitat Pompeu Fabra, Barcelona, Spain
Sánchez del Pino, Manuel (författare)
Biotechnology and Biomedicine Interdisciplinary Research Unit (ERI BIOTECMED), University of Valencia, 46100 Burjassot, Spain
Sancho, Jaime (författare)
ProteoRed-ISCIII, IPBLN -CSIC, 18016 Granada, Spain
Santamaría, Enrique (författare)
Proteored-ISCIII. Proteomics Unit, Clinical Neuroproteomics Group, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, 31008 Pamplona, Spain
Schaeffer-Reiss, Christine (författare)
Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000, Strasbourg, France
Schneider, Justine (författare)
Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, 67000, Strasbourg, France
de la Torre, Carolina (författare)
ProteoRed-ISCIII, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Spain
Valero, M. Luz (författare)
ProteoRed-ISCIII, Proteomics Unit, Central Service for Experimental Research (SCSIE), University of Valencia, 46100, Burjassot, Spain
Vilaseca, Marta (författare)
ProteoRed-ISCIII, Institute for Research in Biomedicine (IRB Barcelona), BIST (The Barcelona Institute of Science and Technology), Baldiri i Reixac 10, 08028 Barcelona, Spain
Wu, Shuai (författare)
ProteoRed-ISCIII, Vall d’Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain; Proteomics and Mass Spectrometry of Biomolecules, Proteomics Infrastructure of Toulouse, Proteomics French Infrastructure, ProFI. Institut de Pharmacologie et Biologie Structurale (IPBS), Universit´e de Toulouse, UPS, CNRS, Toulouse, France
Wu, Linfeg (författare)
ProteoRed-ISCIII, Vall d’Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain; Proteomics and Mass Spectrometry of Biomolecules, Proteomics Infrastructure of Toulouse, Proteomics French Infrastructure, ProFI. Institut de Pharmacologie et Biologie Structurale (IPBS), Universit´e de Toulouse, UPS, CNRS, Toulouse, France
de Embún, Pilar Ximénez (författare)
ProteoRed-ISCIII, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain
Canals, Francesc (författare)
ProteoRed-ISCIII, Vall d’Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain
Corrales, Fernando J. (författare)
ProteoRed-ISCIII, Centro Nacional de Biotecnologia (CSIC), 28049, Madrid, Spain; ProteoRed-ISCIII, CIMA, University of Navarra, 31008, Pamplona, Spain
visa färre...
 (creator_code:org_t)
Elsevier, 2022
2022
Engelska.
Ingår i: Journal of Proteomics. - : Elsevier. - 1874-3919 .- 1876-7737. ; 251
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.

Ämnesord

NATURVETENSKAP  -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Nyckelord

Biochemistry
Biophysics
Biochemistry
biokemi
Analytical Chemistry
analytisk kemi

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Sök utanför SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy