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Ion-mediated change...
Ion-mediated changes of supported lipid bilayers and their coupling to the substrate. A case of bilayer slip?
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- Kunze, Angelika, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fang, Zhao, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Marel, Anna-Kristina (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Svedhem, Sofia, 1970 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kasemo, Bengt Herbert, 1942 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Royal Society of Chemistry (RSC), 2011
- 2011
- Engelska.
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Ingår i: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-6848 .- 1744-683X. ; 7:18, s. 8582-8591
- Relaterad länk:
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Ion-mediated (Ca(2+)) changes in viscoelastic, structural and optical properties of negatively charged solid supported lipid bilayers (SLBs) on SiO(2) surfaces were studied by means of quartz crystal microbalance with dissipation (QCM-D) monitoring and optical reflectometry. Despite the sensitivity of QCM-D to viscoeleastic/structural variations, it has not often been used to probe such changes for SLBs. SLBs were prepared from binary phospholipid mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC, neutral) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG, negatively charged) on SiO(2) sensor surfaces in a Ca(2+)-containing buffer. Interestingly, for bilayers containing POPG fractions above 35%, large QCM-D dissipation shifts occurred, when Ca(2+) was removed from buffer in contact with the SLB (while maintaining 100 mM NaCl). The accompanying frequency changes were small. These Ca(2+) mediated QCM-D responses are reversible, and a signal for considerable changes in the viscoelastic and structural properties of the SLB. Variation of Ca(2+)-concentration revealed a threshold concentration of around 0.4 mM for the changes in the SLB to occur. Below this value, at >35% POPG concentration in the SLB, the SLB appears to become more weakly attached to the SiO(2) substrate, which is partly attributed to a weakening of the POPG-substrate interaction in the absence of Ca(2+). A consequence of this is an oscillation-amplitude dependent dissipation, which we attribute to slip of the bilayer at higher oscillation amplitudes. Complementary experiments using a combined QCM-D/reflectometry instrument showed that the Ca(2+)-induced changes in the viscoelastic/structural properties of the SLB are accompanied by changes in the optical properties. We discuss different scenarios to explain the observed reversible effect of Ca(2+)-ions on the dissipative and optical properties of the mixed SLBs. Based on our results we propose the observed phenomenon to be a combination of geometric changes, internal structural changes, changes in the interfacial water layer, and a slip mechanism, i.e. friction between the SLB and the substrate.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- v513
- reflectometry
- adsorption
- laughlin a
- v39
- 1978
- 1986
- divalent-cations
- p338
- quartz-crystal microbalance
- qcm-d
- atomic-force microscopy
- unilamellar vesicles
- p119
- membranes
- interference-contrast microscopy
- vesicle
- muralis w
- phospholipid-bilayers
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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