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Sökning: onr:"swepub:oai:lup.lub.lu.se:1d17d03a-5ae8-4ae8-a842-7b693c83a463" > Hypersomy of chromo...

Hypersomy of chromosome 15 with retrovirally rearranged c-myc, loss of germline c-myc and IgK/c-myc juxtaposition in a macrophage-monocytic tumour line

Imreh, S (författare)
Wang, Y (författare)
Panda, C K (författare)
visa fler...
Babonits, M (författare)
Axelson, H (författare)
Karolinska Institutet,Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine
Silva, S (författare)
Szeles, A (författare)
Wiener, F (författare)
Klein, G (författare)
visa färre...
 (creator_code:org_t)
Elsevier, 1994
1994
Engelska 9 s.
Ingår i: European Journal of Cancer. - : Elsevier. - 0959-8049. ; 30A:7, s. 994-1002
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • From a lymphoid tumour induced by 7,12-dimethylbenz-[a]-anthracene (DMBA) + methyl-N-nitrose-N-urea (MNU) in an [AKR Rb(6.15) x CBAT6T6]F1 mouse, a macrophage- monocyte line (KT-10) was isolated. Following ethyl methanesulfonate (EMS) treatment, a bromodeoxyuridine (BUdR) resistant subline was selected. Serial propagation of this line in vitro in the presence of BUdR (28 months) with periodic cytogenetic and molecular examinations, has led to the definition of four successive stages. During stage I, the cells were trisomic for chromosome 15. They contained Rb(6.15) and Rb(del6.15) of AKR and T(14;15) of CBA origin. Southern blotting showed the presence of both germline (G) and rearranged (R) c-myc. At stage II, Rb(del6.15) has duplicated. Both Rb(6.15) and T(14;15) persisted together with G-myc and R-myc. In stage III, the CBA-derived T(14;15) was lost, in parallel with G-myc. At this stage, a Dic.In(6.15) was detected. One of its arms was cytogenetically identical with the long arm of In(6.15) in the variant IgK/myc translocations. This chromosome carried R-myc and IgK in juxtaposition, as indicated by comigration on pulsed field electrophoresis (PFGE). At stage IV, the R-myc carrying AKR-derived chromosome 15s were present in six copies. Possible relationships between the increasing R/G myc ratio and changed growth characteristics in vivo and in vitro are discussed.

Nyckelord

9,10-Dimethyl-1,2-benzanthracene
Animals
Blotting, Northern
Blotting, Southern
Chromosome Aberrations
Chromosomes, Human, Pair 15
Electrophoresis, Gel, Pulsed-Field
Genes, myc
Humans
Lymphoma, T-Cell
Macrophages
Methylnitrosourea
Mice
Mice, Inbred AKR
Mice, Inbred BALB C
Monocytes
Trisomy
Tumor Cells, Cultured

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