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Human skeletal musc...
Human skeletal muscle cell differentiation is associated with changes in myogenic markers and enhanced insulin-mediated MAPK and PKB phosphorylation
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- Al-Khalili, L (författare)
- Karolinska Institutet
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- Krämer, D (författare)
- Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden; Dept. of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
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- Wretenberg, Per, 1963- (författare)
- Karolinska Institutet,Region Örebro län,Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden
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- Krook, A (författare)
- Karolinska Institutet
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Karolinska Institutet Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden; Dept of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden (creator_code:org_t)
- Wiley-Blackwell, 2004
- 2004
- Engelska.
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Ingår i: Acta Physiologica Scandinavica. - : Wiley-Blackwell. - 0001-6772 .- 1365-201X. ; 180:4, s. 395-403
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- AIM: We hypothesized that myogenic differentiation of HSMC would yield a more insulin responsive phenotype.METHODS: We assessed expression of several proteins involved in insulin action or myogenesis during differentiation of primary human skeletal muscle cultures (HSMC).RESULTS: Differentiation increased creatine kinase activity and expression of desmin and myocyte enhancer factor (MEF)2C. No change in expression was observed for big mitogen-activated protein kinase (BMK1/ERK5), MEF2A, insulin receptor (IR), hexokinase II, and IR substrates 1 and 2, while expression of glycogen synthase, extracellular signal-regulated kinase 1 and 2 (ERK1/2 MAP kinase) and the insulin responsive aminopeptidase increased after differentiation. In contrast to protein kinase B (PKB)a, expression of (PKB)b increased, with differentiation. Both basal and insulin-stimulated PI 3-kinase activity increased with differentiation. Insulin-mediated phosphorylation of PKB and ERK1/2 MAP kinase increased after differentiation.CONCLUSION: Components of the insulin-signalling machinery are expressed in myoblast and myotube HSMC; however, insulin responsiveness to PKB and ERK MAP kinase phosphorylation increases with differentiation.
Ämnesord
- SAMHÄLLSVETENSKAP -- Psykologi (hsv//swe)
- SOCIAL SCIENCES -- Psychology (hsv//eng)
Nyckelord
- Akt; protein kinase B; cell culture; ERK mitogen-activated protein kinase
- Psykologi
- Psychology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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