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Epitope specificity...
Epitope specificity of monoclonal anticytokeratin antibody TS1
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Johansson, A (författare)
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Sandström, P (författare)
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Ullén, A (författare)
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Behravan, G (författare)
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Ärlestig, L (författare)
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- Sundström, Birgitta (författare)
- Karlstads universitet,Avdelningen för kemi och biomedicinsk vetenskap
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- Erlandsson, Ann (författare)
- Karlstads universitet,Avdelningen för kemi och biomedicinsk vetenskap
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Levi, M (författare)
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Stigbrand, T (författare)
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visa färre...
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(creator_code:org_t)
- 1999
- 1999
- Engelska.
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Ingår i: Cancer Res 1999;59:45-51.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Due to their abundance in epithelial cells and deposition in necrotic regions intratumorally, cytokeratins (CKs) have been established as valuable targets for both radioimmunolocalization and radioimmunotherapy. The target epitope for the monoclonal anti-CK8 antibody, TS1, used for both experimental radioimmunolocalization and radioimmunotherapy, was determined by means of synthesis of 96 overlapping peptides that covered the entire CK8 molecule. A highly conserved peptide sequence, spanning amino acids (aa) 343357 and covering the discontinuous epitope in the helical 2B domain, was identified. The epitope retains its helical structure, as shown with circular dichroism spectroscopy, although the length of the peptide (i.e., >20 aa) is crucial for maintenance of immunoreactivity. To determine which aa residues are crucial for binding to the monoclonal antibody, alanine scanning was performed on a 26-mer covering aa 340365, with the sequence RGELAIKDANAKLSELEAALQRAKQ. The 26 modified peptides were evaluated using ELISA and BIAcore technology. The uniqueness of this epitope has been established by data base sequence comparisons
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- Biomedicin
- Biomedical Science
- Biomedical Laboratory Science
- Biomedicinsk laboratorievetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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